Introducing Toyopearl Q-600C AR: a new high capacity resin for downstream purification

Toyopearl Q-600C AR is a new alkaline resistant (AR) resin for the large scale purification of proteins. It has a particle size of 100µm with excellent flow properties for the capture step in manufacturing processes. It maintains its dynamic binding capacity (DBC) even after repeated exposure to or storage in caustic solutions of concentrations up to 1mol/L NaOH. This allows the new resin to withstand multiple clean-in-place (CIP) cycles and greatly extends its useful life for the process step. Toyopearl Q-600C AR resin has typically 3 times the dynamic binding capacity of Toyopearl QAE-550C resin for smaller proteins such as BSA, and can also be used with proteins as large as thyroglobulin (660kDa). For more information on the caustic stability, DBC values for various proteins, its breakthrough curve, or additional comparison details, please see the product page.

Application Notes new to our library: AN21: Improved Reproducibility of MW Measurements of Polypeptoids using an EcoSEC® GPC System The EcoSEC GPC System and a set of TSKgel mixed-bed columns were used successfully for obtaining high quality MWD data of a series of block poly-ß-alkylalanoids with HFIP as the mobile phase in under 15 minutes. Because of excellent flow rate and temperature control, and baseline stability of the EcoSEC GPC System, average MW values ranged from 0.2 to 0.3% relative standard deviation, a ten-fold reproducibility improvement as compared to SEC literature data of polyamides using a similar mobile phase system. AN22: High Throughput Reversed Phase HPLC Analysis of Caffeine using TSKgel ODS-140HTP and ODS-100V Columns HPLC methods are commonly used for the analysis of caffeine in beverages. The successful analysis of caffeine utilizing two different TSKgel reversed phase columns, a TSKgel ODS-140HTP, 2.3µm column and a TSKgel ODS-100V, 5µm column is reported. New Technical Presentations added to our library: TP143: High Throughput Analysis of Caffeine and Other Food and Beverage Additives Present in Popular Soft Drinks using High Performance Reversed Phase HPLC More detailed information on Application Note 22 (discussed above) that was presented at the recent ACS Fall Meeting. TP144: High Throughput Analysis of a Number of Common Drugs using a TSKgel ODS-140HTP, 2.3µm Column This poster presented at the ACS Fall Meeting reports the analysis of a number of drugs coming off patent in 2009 and in the coming years using a TSKgel ODS-140HTP column. Lamotrigine (generic name Lamictal) could be separated with low retention time (< 2 minutes), good symmetry and high theoretical plate values with a TSKgel ODS-140HTP column. New columns for ultra-high MW (>105) polymer analysis: TSKgel GMHhr-H columns are now available in larger particle sizes of 13, 20 and 30µm, while a 13µm TSKgel GMHhr-M(S) column now complements the 5µm mixed-bed TSKgel GMHhr-M column. These large particle size mixed-bed columns overcome the effects of shearing that often occur as extremely large polymers move through a column of small particle size (as shown in the chromatograms below).

See us at these upcoming shows: ISPPP Delray Beach, FL October 25-28 Booth #7 EAS Somerset, NJ November 16-19 Booth #219 Introducing Joe Machamer: Join us in welcoming Joe to the GPC team here at Tosoh Bioscience. He is our new GPC Marketing Manager. Contact Joe now for additional information or with questions on our EcoSEC GPC System. Save Money: Column Promotions     1. Savings + Extend the lifetime of your column and receive a 25% discount! Save 25% on any TSKgel guard product when ordered online. 2. More Affordable GPC Separations! Save 25% on all TSKgel H-type GPC columns. 3. Savings for educational and governmental institutions! Save 15% on all HPLC columns and laboratory size resin containers (< 1L). EcoSEC GPC System Go Green, Get Green Promotion Go Green: Lower solvent consumption and increased throughput make this system environmentally and budgetary friendly. Get Green: Receive a trade-in discount (up to $10,000) with your EcoSEC GPC System instrument purchase.

Tosoh Bioscience, TSKgel and Toyopearl are registered trademarks of Tosoh Corporation.