Unlike other TSK-GEL affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.  The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.

 Applications include: glycoproteins, antigens, etc.

Structure:

Product Attributes:

Pore size (mean): 1000Å  Particle size (mean): 10µm  pH stability: 2.0 - 12.0  Ligand conc.: ca. 20µmol/mL resin  Adsorption capacity: > 60mg/g dry resin (coupling capacity w/soybean trypsin inhibitor)  Exclusion limit  (base resin, estimate): < 10,000,000 Da globular proteins  Active group:  tresyl

Literature:

Catalog -- Chromatography Catalog, AFC Columns

Operating Conditions and Specifications 

Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns

Brochure -- TSK-GEL HPLC Columns for Biomolecular Applications