Affinity Chromatography (AFC) offers the greatest potential specificity and selectivity for the isolation or purification of biomolecules.  To enhance this advantage, three group specific ligands and one chemically active functionality are available through Tosoh Bioscience.

All analytical TSK-GEL AFC columns are based on the well-established 10 micron rigid TSKgel G5000PW resin. This resin features 1000Å pores that have an estimated exclusion limit of < 10 million Da along with excellent stability from pH 2 to 9.

 

Group Specific Ligands	Application
TSKgel ABA-5PW	Serine and trypsin-like serine protease inhibitors
TSKgel Boronate-5PW	Cis-diol groups found on carbohydates, glycosolyated proteins, catecholamines
TSKgel Chelate-5PW	Chelates with Zn+2, Cu+2 and Ni+2 ions to target  histidine residues on proteins
TSKgel Heparin-5PW	Blood clotting factors, glycoproteins, endoglycosidase, hyaluronidase, lipases, growth factors, nucleic acid-binding proteins
Activated Ligands	Application
TSKgel Tresyl-5PW	Reacts with amino or thiol groups, used primarily for attachment of specific antigens for antibody analysis