Common Characteristics of TSK-GEL Anion and Cation Exchangers

The methacrylate base resin, G5000PW (5PW), is a spherical 10μm particle with approximately 1000Å pores. TSK-GEL 5PW is derivatized with diethylaminoethyl (DEAE), sulfopropyl (SP) or carboxymethyl (CM) functionalities to provide a weak anion, a strong cation, and a weak cation exchanger, respectively. The polyamine chemistry employed in TSKgel SuperQ-5PW results in a high capacity strong anion exchanger with a smaller effective pore size than TSKgel DEAE-5PW. Particles in TSKgel BioAssist Q columns contain very large pores (~4000Å) that are functionalized with polyamine groups to form a network structure. The capacity of BioAssist Q columns is high over a wide molecular weight range. TSK-GEL BioAssist S columns are packed with particles possessing 1300Å pores functionalized with sulfopropyl groups. Methacrylate is also the backbone of nonporous resin (NPR) columns such as DEAE-NPR, DNA-NPR and SP-NPR, which are packed with 2.5μm particles. High column efficiency coupled with low sample capacity restricts the application of these columns to fast analysis and micro-scale preparative isolation. Due to the absence of large pores, protein recovery is generally very high on NPR columns.  Anion Exchange Columns (type, matrix)  Cation Exchange Columns (type, matrix) Strong anion, methacrylic TSKgel BioAssist Q TSKgel SuperQ-5PW Strong anion, silica TSKgel QAE-2SW Strong anion, polystyrene TSKgel Sugar AXI TSKgel Sugar AXG TSKgel SAX Weak anion, methacrylic TSKgel DEAE-5PW TSKgel DEAE-NPR TSKgel DNA-NPR Weak anion, silica TSKgel DEAE-2SW TSKgel DEAE-3SW Strong cation, methacrylic TSKgel BioAssist S TSKgel SP-5PW TSKgel SP-NPR Strong cation, silica TSKgel SP-2SW Strong cation, polystyrene TSKgel SCX Weak cation, methacrylic TSKgel CM-5PW TSKgel OApak-A Weak cation, silica TSKgel CM-2SW TSKgel CM-3SW