Starting in the mid 1970s Reversed Phase Liquid Chromatography (RPC) has become the standard technique to analyze small molecular weight compounds in industrial, academic and governmental laboratories.

More recently, RPC has become the accepted tool for the separation of peptides, proteins and other biopolymers in the pharmaceutical, chemical and biochemical industries.

Tosoh Bioscience offers 15 distinct Reversed Phase column types which are either based on silica or methacrylate particles as discussed below.

Silica-based Columns

Methacrylate-based Columns

The silica-based TSK-GEL reversed phase column products consist of eleven stationary phases over 4 key product families. Each TSK-GEL silica column features: High purity Type B Silica HIgh efficiencies  Excellent recoveries Low bleed for MS An excellent choice for analysis of small molecules and peptides. TSK-GEL silica-based reversed phase columns are grouped into 4 product families: New ODS-100V and 100Z (100Å) Monomeric Bonded Silica (80Å) High Efficiency (110Å) Specialty Silica Columns The polymeric-based TSK-GEL reversed phase columns are synthesized from polymethacrylate polymers in a range of pore and particle sizes. Advantages of polymethacrylate particles include: Hydrophilic backbone to improve recovery and reduce secondary interactions pH stable from 1 to 12 Compatibility with organic solvents eliminates swelling An excellent choice for large MW biomolecules (>10,000 Da) and for analyzing small MW compounds at high pH. TSK-GEL polymeric reversed phase columns are offered in 4 different chemistries: Octadecyl-2PW (125Å) Octadecyl-4PW (500Å) Phenyl-5PW RP (1000Å) Octadecyl-NPR (nonporous)