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TSK-GEL Super-ODS, Super-Octyl and Super-Phenyl columns are prepared from spherical 2µm silica particles with 140Å pore diameter. The silica beads in the columns are extremely uniform in size which provides column efficiencies of more than 160,000 plates per meter, yet have a pressure drop comparable to columns packed with 3µm particles. The main advantages of the Super Series columns are savings in time and solvent usage, in addition to the improved quantitation resulting from reduced band broadening.
Caution: The silica particles in Super Series columns have a relatively small pore volume, which results in shorter retention times than obtained on most other reversed phase columns. For instance, to achieve similar retention times as obtained on TSKgel ODS-100V, the percentage organic solvent in the mobile phase has to be reduced by about 20-30% on TSKgel Super-ODS columns. |
Highlights
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The silica particles used in Super Series columns are uniform spherical 2µm beads with 140Å pores.
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Pressure drop is not excessive due to the monodisperse particle size distribution.
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Polymeric bonded phase structure and exhaustive endcapping effectively shields the solutes from interacting with residual silanol groups.
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TSK-GEL Super Series require approximately 20% less organic solvent than other popular TSK-GEL reversed phase columns.
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2µm particles provide superior resolution and speed, as well as improved sensitivity. |