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Tosoh Bioscience LLC: The Chemistry of Innovation
Gel Permeation Chromatography
Alpha
SuperAW
TSKgel SuperAW2500
TSKgel SuperAW3000
TSKgel SuperAW4000
TSKgel SuperAW5000
TSKgel SuperAW6000
TSKgel SuperAWM-H


TSK-GEL SuperAW columns are composed of spherical, hydrophilic methacrylate beads. Figure 1 shows the calibration curves of all TSK-GEL SuperAW columns.

Figure 1: Polyethylene oxide (PEO), polyethylene glycol (PEG) and Ethylene glycol (EG) calibration curves for TSK-GEL SuperAW series columns



Unlike PW/PWxl-type columns, which are stable up to 50% organic mixed with water, SuperAW and Alpha-type columns are stable in a wide variety of organic solvents at concentrations up to 100%. As shown in Figure 2, efficiency of all SuperAW-type columns is maintained when changing solvents from water via acetonitrile, DMF, DMSO, THF to HFIP.

Figure 2: Solvent Compatibility of TSK-GEL SuperAW Series



TSK-GEL SuperAW columns are used for high efficiency and high speed analysis of polymers that are soluble in polar organic solvents. Examples include cleansing gel in methanol, degree of saponification of polyvinylalcohol in HFIP, and sodium dodecylsulfate, polyimide, and cellulose derivatives in DMF with 10mmol/L LiBr.

Mixed-bed TSK-GEL SuperAW Column
The  mixed-bed column,
TSKgel SuperAWM-H, has an extended linear calibration range, and is suitable for samples with a broad MW distribution as well as samples with unknown molecular weight.


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Application Overview -- Gel Filtration Chromatography with TSK-GEL columns

EcoSEC GPC System main page

Tosoh Bioscience LLC  156 Keystone Drive, Montgomeryville, PA, 18936  Tel: (215) 283-5000  Email: info.tbl@tosoh.com