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TSK-GEL SWxl columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.
Particle Characteristics
TSK-GEL SWxl-type columns contain smaller particles than SW-type columns; 5 and 8μm versus 10 and 13μm. As the SW-type columns, TSK-GEL SWxl columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSK-GEL SWxl-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.
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Calibration Curves
The Figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSK-GEL SWxl-type columns. The following Table summarizes the molecular weight ranges for G2000SWxl, G3000SWxl and G4000SWxl columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSK-GEL SW and SuperSW-type columns. |
Protein Calibration curves for
TSK-GEL SWxl columns
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Properties and separation ranges for TSK-GEL SW-type packings (Molecular Weight in Da)
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TSK-GEL packing |
Particle
size (µm) |
Pore
size (Å) |
Globular
proteins |
Dextrans |
Polyethylene
glycols and oxides |
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SuperSW2000 |
4 |
125 |
5,000 - 1.5 x 10^5 |
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G2000SWxl
BioAssist G2SWxl |
5 |
125 |
5,000 - 1.5 x 10^5 |
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QC-PAK TSK200 |
5 |
125 |
5,000 - 1.5 x 10^5 |
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G2000SW |
10, 13 |
125 |
5,000 - 1 x 10^5 |
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SuperSW3000 |
4 |
250 |
1 x 10^4 - 5 x 10^5 |
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G3000SWxl
BioAssist G3SWxl |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
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QC-PAK TSK300 |
5 |
250 |
1 x 10^4 - 5 x 10^5 |
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G3000SW |
10 |
250 |
1 x 10^4 - 5 x 10^5 |
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G4000SWxl
BioAssist G4SWxl |
8 |
450 |
2 x 10^4 - 7 x 10^6 |
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G4000SW |
13, 17 |
450 |
2 x 10^4 - 7 x 10^6 |
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Data generated using the following conditions:
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Columns: |
Two 4µm, 4.6mmIDx30cm TSK-GEL SuperSW columns in series; two 5µm, 7.8mmIDx30cm TSK-GEL SWxl columns in series; two 10µm, 7.5mmIDx60cm TSK-GEL SW columns in series |
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Elution: |
Globular proteins: 0.3M NaCl in 0.1M (0.05M for SWxl columns) phosphate buffer, pH 7
Dextrans and polyethylene glycols and oxides (PEOs): distilled water |
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Application Overview -- Gel Filtration Chromatography with TSK-GEL columns |