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Tosoh Bioscience LLC: The Chemistry of Innovation
Size Exclusion: Gel Filtration Chromatography
Proteins
Water-soluble Polymers
PW
PWxl
PWxl-CP for Cationic Polymers
Polar Organic-soluble Polymers
Organic-soluble Polymers


TSK-GEL PWxl- and PW-type columns are the columns of choice for analyses of water-soluble polymers.  All TSK-GEL polymer-based gel filtration columns are prepared from polymethacrylate resin.

PWxl- and PW-type

  • Use higher efficiency PWxl-type columns for optimal resolution, to reduce analysis time, or in sample limited applications.
  • Use PW-type columns when analysis time is not critical, when sample mass is not limited, to collect fractions, or to obtain maximum number of plates (at the expense of analysis time).

PWxl-CP columns

  • PWxl-CP columns have been chemically modified to improve recovery of cationic polymers.

Highlights:

  • PW, PWxl and PWxl-CP columns
    • Hydrophilic methacrylate beads.
    • Particle sizes are 7-13μm for PWxl and PWxl-CP columns, and 12-17μm for PW-type. 
    • Higher % crosslinking for smaller particle size PWxl-type resins.
    • Stable from pH 2 to 12
    • Eluent: water or buffer (up to 20% methanol/80% aqueous)
    • G6000PW is available in PEEK column hardware for ultra-low sample adsorption during virus analysis.
    • Temperatures up to 80˚C (50˚C for TSKgel G-DNA-PW).
  • PWxl-CP columns
    • Minimal adsorption of cationic polymers.

TSK-GEL PW and PWxl

TSK-GEL PWxl-CP

As TSK-GEL PW-type columns, there are seven TSK-GEL PWxl-type columns varying in pore size from less than 100Å to more than 1000Å. Mixed-bed columns are also available.

The TSK-GEL PWxl product line also offers specialty columns for analyzing:

TSK-GEL PWxl-CP columns have the same base resin as the PWxl columns and were specifically developed for the analysis of water-soluble cationic polymers and allow elution under low salt conditions.

Avaliable PWxl-CP-type columns and pore sizes:

  • G3000PWxl-CP  [200Å]
  • G5000PWxl-CP  [1000Å]
  • G6000PWxl-CP  [>1000Å]

 


Return to Size Exclusion main

Application Overview -- Gel Filtration Chromatography with TSK-GEL columns

 

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TSK-GEL PWxl-CP columns for water-soluble cationic polymer analysis
      
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Tosoh Bioscience LLC  156 Keystone Drive, Montgomeryville, PA, 18936  Tel: (215) 283-5000  Email: info.tbl@tosoh.com