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TSK-GEL PW columns are composed of spherical, hydrophilic polymethacrylate beads. Particle sizes range from 12µm for the smaller pore size columns to 17µm for the larger pore size columns.
The main application areas for PW columns are the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides.
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The figure to the right shows the calibration curves for all PW-type columns. Suitable solvents for various polymer samples are shown in the table below.
Specialty PW-type Columns
The mixed-mode column, TSKgel GMPW, has an extended linear calibration range, suitable for samples with a broad MW distribution, as well as unknown samples.
TSKgel G2000PW, the larger particle size equivalent of TSKgel G-Oligo-PW, is most suitable for semipreparative and preparative isolation of oligosaccharides.
TSKgel G1000PW is the preferred column for analysis of low MW oligomers. |
Polyethylene glycol and oxide calibration curves for TSK-GEL PW columns
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Recommended eluents for GFC of water-soluble polymers on TSK-GEL PW-type columns
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Type of polymer |
Typical sample |
Suitable eluent |
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Nonionic hydrophilic |
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soluble starch, methyl cellulose, pullulan |
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dextran, hydroxyethyl cellulose |
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polyvinyl alcohol, polyacrylamide |
Buffer or salt solution (e.g. 0.1-0.5M NaNo3) |
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Nonionic hydrophobic |
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Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1M NaNO3) |
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Anionic hydrophilic |
sodium chondroitin sulfate, sodium alginate, carboymethyl cellulose, sodium polyacrylate, sodium hyaluronate |
Buffer or salt solution (e.g. 0.1M NaNO3) |
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sulfonated lignin sodium salt, sodium polystyrenesulfonate |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1M NaNO3) |
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Cationic hydrophilic |
glycol chitosan, DEAE-dextran, poly(ethyleneimine), poly(trimethylaminoethyl methacrylate) iodide salt |
0.5M acetic acid with 0.3M Na2SO4 or 0.8M NaNO3 |
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Cationic hydrophobic |
poly(4-vinylbenzyltrimethylammonium chloride), poly(N-methyl-2-vinylpyridinium) iodide salt |
0.5M acetic acid with 0.3M Na2SO4 |
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Amphoteric hydrophilic |
peptides, proteins, poly- and oligosaccharides, DNA, RNA |
Buffer or salt solution (e.g. 0.1M NaNO3) |
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Amphoteric hydrophobic |
blue dextran, collagen, gelatin, hydrophobic proteins, hydrophobic peptides |
Buffer or salt solution with organic solvent (e.g. 20% CH3CN in 0.1M NaNO3 or 35-45% CH3CN in 0.1% TFA) | |
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Application Overview -- Gel Filtration Chromatography with TSK-GEL columns |