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Tosoh Bioscience LLC: The Chemistry of Innovation
Ion Exchange Chromatography
Ion Exchange Chromatography
Anion Exchange
Cation Exchange
TSKgel BioAssist S
TSKgel CM-2SW
TSKgel CM-3SW
TSKgel CM-5PW
TSKgel CM-STAT
TSKgel OApak-A
TSKgel SCX
TSKgel SP-2SW
TSKgel SP-5PW
TSKgel SP-NPR
TSKgel SP-STAT


Cation exchange chromatography is practiced with either a strong or a weak cation exchange column, containing a sulfonium ion, or with a weak cation exchanger, having usually a carboxymethyl (CM) functional group. A counter ion, often Na+ maintains electroneutrality.

In ion exchange chromatography the pH of the mobile phase buffer must be between the pI or pKa of the charged molecule and the pKa of the charged groups on the solid support. For example, a molecule with a pI of 8.2 is run in a mobile phase buffer at pH 6.0 with the solid support pKa at 1.2 in cation exchange chromatography.

Specialty columns:

Highlights

  • Pore structure and bonding chemistry of TSKgel BioAssist S provides high capacity for medium to large molecular weight proteins.
  • TSKgel BioAssist columns are packed in 4.6mmID or 10mmID PEEK hardware.  Other columns are available in glass and stainless steel for anlytical, semi-preparative and preparative applications.
  • Binding capacity  for small to medium size proteins on TSKgel DEAE-3SW is approximately double that of the 5PW packings due to the smaller pore size and larger surface area.
  • TSKgel SP-5PW column available in 2mmID format.
  • Very efficient chromatography can be achieved with the TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores.  


Strong Cation Exchange 

Weak Cation Exchange

Polymethacrylate:

Silica: TSKgel SP-2SW

Polystyrene: TSKgel SCX

Polymer: TSKgel SP-STAT

Polymethacrylate:

Silica:

Polymer: TSKgel CM-STAT

 


Contact us:

Tech Service:
800-366-4875 (option #3)

Customer Service:
866-527-3587

info.tbl@tosoh.com
 


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TSKgel CM-STAT
TSKgel SP-STAT 
Cation Exchange Columns 

Packed with non-porous resin particles, these columns enable high speed and high resolution analysis, as well as isolation, of biomolecules.


Cation Exchange Chromatography is performed with either a strong cation exchanger, containing a bonded sulfonic acid group, such as sulfopropyl (SP), or with a weak cation exchanger, containing a weak acid such as carboxymethyl (CM). The advantage of strong vs. weak ion exchangers is that the first are charged over a wider pH range. Weak ion-exchangers often provide slightly different selectivity from strong exchangers. 

Tosoh Bioscience LLC 3604 Horizon Drive, Suite 100, King of Prussia, PA, 19406 Tel: (800) 366-4875  Email: info.tbl@tosoh.com