TSKgel BioAssist S columns are packed with porous hydrophilic polymer beads which are surface modified with a strong cation exchanger. The resin's large pores are very accessible even for high molecular weight proteins. This leads to higher chromatographic efficiency and binding capacity for purification.

The application on this page shows the analysis of bromelain, a proteolytic enzyme that is used as a nutritional supplement. Bromelain is a basic glycoprotein with a MW of 33kDa and pI of 9.55.

Product Attributes:

Matrix: polymethacrylate  Particle size (mean): 7µm and 13µm  Pore size (mean): 1,300Å  Functional group: sulfopropyl  Counter ion: Na+  pH range: 2.0 -12.0  Capacity (gamma globulin) 70  Small ion capacity: 0.1 meq/mL  pKa: 2.4

Literature:

Application Note -- Ion Exchange Analysis of Monoclonal Antibodies on Large Pore TSKgel BioAssist Columns

Application Note -- Separation of IgM using TSKgel BioAssist S

Operating Conditions and Specifications

Product Overview -- TSKgel BioAssist Ion Exchange Columns

Separation Report - TSKgel BioAssist Series Ion Exchange Columns

Technical Presentation -- Purification of Immunoglobulin M using a novel ion-exchanger

Technical Presentation -- The Characteristics of Novel Semi-Preparative Ion-Exchange Columns

Technical Presentation -- Fast Protein Separations with Ion Exchange Columns