TSKgel NH2-100 amino columns expand the range of TSK-GEL columns for hydrophilic interaction chromatography (HILIC). Offering a different selectivity from the well known TSK-GEL Amide-80 columns, these novel amino-bonded phase columns stand out by providing much improved chemical stability, a prerequisite for achieving reproducible and reliable results.
TSK-GEL NH2-100 columns are packed with 3µm silica particles containing 100Å pores. A novel bonding strategy was adopted to improve chemical stability of the bonded phase. First the silica is reacted with a trimethylsilane endcapping reagent at a low stoichiometric ratio, before reacting residual and accessible silanol groups with trifunctional alkylaminosilane reagent. The resulting bonded phase provides a better safeguard against hydrolysis of the underlying silica.
TSK-GEL NH2-100 columns are unique in that the bonded phase ligand not only, as expected, has a terminal primary amino group, but that the spacer also incorporates secondary as well as tertiary amino groups. Anionic compounds are retained on the column by ionic interaction. This allows for the use of gradients in salt concentration, in addition to gradient elutions with acetonitrile. Since the TSK-GEL NH2-100 columns have cationic sites, the columns can be used as mixed mode columns under some conditions.
TSK-GEL NH2-100 columns are well suited for the analysis of all types of hydrophilic compounds, including carbohydrates and peptides. Due to a high ligand density and large surface area, these columns show stronger retention of polar compounds than TSK-GEL Amide-80 columns.
|