The variation between different lots of TSKgel ODS-80Ts QA packing material is minimized by selecting batches of TSKgel ODS-80Ts that fall within a very narrow range of specifications, as demonstrated in the table below. In addition, each column must pass demanding specifications for efficiency (N) and peak asymmetry, as are spelled out in the Operating Conditions and Specifications sheet. The end result is TSKgel ODS-80Ts QA columns that exhibit an unparalleled level of reproducibility for retention, selectivity (k´), efficiency (N), and peak symmetry.

Product Specifications:

Attribute Specification Range Lot-to-Lot Reproducibility (CV%) Particle size: - Distribution (dp90/dp10) 4.95 - 5.35 1.55 - 1.70 0.6 1.8 Surface area (m2/g) 410 - 440 0.5 Pore size (mL/g) silica: 9.0 - 10.0 0.5 Pore volume (nm): 0.96 - 1.04 0.7 Carbon content (wt%): 14.0 - 15.0 N/A C18 coverage (µmol/m2): 1.71 - 1.99 1.3 Metal ion content (ppm) - Na - Al - Fe - Ti <10 <10 <10 <10 N/A N/A N/A N/A

Column classification:

TSKgel ODS-80Ts QA columns were submitted to several characterization tests to determine the level of hydrophobic retention, steric selectivity and retention of basic compounds. The results of the characterization tests were used to establish specifications listed in the Table below.

Parameter Specification CV (%) Test Conditions k' naphthalene (hydrophobicity) 1.53 - 1.63 1.3 1 α triphenylene/o-terphenyl (steric selectivity) 1.21 - 1.25 0.4 1 k' procainamide (basic compounds) 1.35 - 1.55 2.6 2 k' phenol 9.25 - 9.85 0.9 2 k' oxine copper (inertness to chelating compounds) 1.13 - 1.35 3.5 3

1. 80% CH₃OH/20% H₂O, T=40°C
2. 10% ACN/90% 20mmol/L Na₂HPO₄ (pH6.6, adjusted with 20mmol/L NaH₂PO₄, T=40°C)
3. 7% ACN/93% 20mmol/L H₃PO₄, T=40°C

Application To demonstrate the lot-to-lot reproducibility that can be expected when using TSKgel ODS-80Ts QA columns, the contents of an over-the-counter analgesic pain reliever was analyzed on columns from three different lots of packing material. The results are shown in the Figure to the right and Table below. Excellent reproducibility of retention, peak height, peak area, efficiency and peak shape is evident for all three ingredients. Highly reproducible results can be achieved using the TSKgel ODS-80Ts QA columns as shown in this lot-to-lot reproducibility test.

Analysis of an over-the-counter analgesic pain reliever

Results

Compound Lot# RT (min) Peak Area (mv x sec) Peak Height N AF caffeine 01W 02W 03W 3.047 3.080 3.080 4.51575 x 102 4.50235 x 102 4.44175 x 102 99.26 94.04 95.63 10,396 9,597 10,143 1.10 1.13 1.13 salicylamide 01W 02W 03W 6.190 6.253 6.250 3.96172 x 102 3.94822 x 102 3.90617 x 102 54.06 52.23 52.40 16,441 15,797 16,333 1.03 1.07 1.06 acetylsalicylic acid 01W 02W 03W 9.983 10.080 10.063 4.34473 x 102 4.33835 x 102 4.27633 x 102 37.35 36.48 36.28 16,742 16,297 16,649 1.03 1.05 1.05

Literature:

Catalog -- Reversed Phase Columns, silica-based

Operating Conditions and Specifications

Ordering information:
To place an order please click on the shopping cart next to the desired item, or contact Customer Service. Tosoh Bioscience LLC only accepts orders from customers in North and South America. For sales offices in other parts of the world, please click Locations.

P/N Description Particle size Housing material ID (mm) Length (cm) Price ($) 18768 TSKgel ODS-80Ts QA 5μm Stainless Steel 2 15 428 18769 TSKgel ODS-80Ts QA 5μm Stainless Steel 2 25 479 18519 TSKgel ODS-80Ts QA 5μm Stainless Steel 4.6 7.5 443 18520 TSKgel ODS-80Ts QA 5μm Stainless Steel 4.6 15 496 18521 TSKgel ODS-80Ts QA 5μm Stainless Steel 4.6 25 498 19325 Guard Cartridge for 2mm ID columns, 3 pk 5μm Stainless Steel 2 1 333 19308 Guard Cartridge Holder for 2mm ID cartridges Stainless Steel 2 1 324 19011 Guard Cartridge for 4.6mm ID columns, 3 pk 5μm Stainless Steel 3.2 1.5 241 19018 Guard Cartridge Holder for 4.6mm ID cartridges Stainless Steel 3.2 1.5 325