Featuring trimethylsilane groups covalently attached to large 250Å pores, TSKgel TMS-250 is an excellent choice for analysis of larger proteins by reversed phase HPLC. Due to the low hydrophobicity of the ligand, excellent recoveries are common even when used with large proteins. Proteins such as adolase (158kDa) exhibit sharp peaks relative to wide pore C18 columns.

Structure:

Product Attributes:

Pore size (mean): 250Å  Exclusion Limit: 200,000 Da  Endcapped: Yes  Particle size (mean): 10µm  pH stability: 2.0 - 7.5  Functional group: C1 (monomeric bonding chemistry)  % carbon 5%

Literature:

Catalog -- Reversed Phase Columns, silica-based

Operating Conditions and Specifications