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Tosoh Bioscience LLC: The Chemistry of Innovation
TSKgel Columns
Size Exclusion Chromatography
Affinity
Hydrophobic Interaction
Ion Exchange
Normal Phase / HILIC
Reversed Phase
Size Exclusion
Proteins
Water-soluble Polymers
Polar Organic-soluble Polymers
Organic-soluble Polymers
High Temp GPC Columns
Custom Columns
Parts and Accessories


Size Exclusion Chromatography (SEC) is the dominant mode of separation for polymers. It is based on the discrimination of individual sample components by the pores of the packing material.
Large sample molecules cannot or can only partially penetrate the pores, whereas smaller molecules can access most or all pores. Thus, large molecules elute first, smaller molecules elute later, while molecules that can access all the pores elute last from the column.

SEC is usually called Gel Filtration Chromatography when working with water-soluble polymers, while Gel Permeation Chromatography is preferred when working with polymers soluble in polar organic or organic solvents.

Gel Filtration Chromatography for
Proteins and Water-Soluble Polymers

Gel Permeation Chromatography for
Polar and Organic-Soluble Polymers

GFC is popular among biochemists for the isolation of proteins, for the removal of aggregates, to desalt a protein sample, to separate nucleic acid fractions, or to characterize water-soluble polymers used in food products, paints, pharmaceutical preparations, etc.

Avaliable TSKgel products are classified by application area and particle composition.

Select a type to learn more about the various product groups within each type.

Silica

  • SW, SWxl and SuperSW columns for proteins and nucleic acids using an aqueous buffer as mobile phase
  • BioAssist DS columns for desalting of proteins.
  • NEW specialized SW mAb columns for the separation and analysis of monoclonal antibodies (mAb). 

Polymethacrylate

  • Application Area: water-soluble polymers
  • PW and PWxl columns for industrial polymers, oligosaccharides, nucleic acids, small viruses using aqueous buffer or salt solutions as mobile phase
  • PWxl-CP columns for water-soluble cationic polymers using low salt conditions during elution
  • SuperMultiporePW columns are packed with particles containing a broad range of pore sizes enabling high speed and high resolution analysis

GPC plays an important role in the characterization of polar organic-soluble and organic-soluble polymers in consumer, chemical and petrochemical industries.

Polymethacrylate (high % X-linking)

  • Sample: polar organic-soluble polymers
  • Alpha and SuperAW columns for industrial polymers soluble in many polar organic solvents ranging from methanol, acetonitrile, DMF, DMSO, to THF and HFIP.

Available column types for organic-soluble polymers are grouped according to their relative lack of adsorptive properties and speed of analysis. High throughput column dimensions are 4.6 or 6mm ID x 15cm, while conventional columns are 7.8mm ID x 30cm.

Polystyrene

  • Application Area: organic-soluble polymers
  • Ultra-low adsorption columns with limited range of solvents
    • SuperHZ and SuperMultiporeHZ columns (High Throughput)
    • Hxl and Multipore columns (conventional)
  • Low adsorption columns with expanded solvent range
    • SuperH (High Throughput)
    • Hhr (conventional)
  • High temperature columns
    • Up to 140ºC
    • Up to 220ºC



EcoSEC GPC System main page


Size exclusion chromatography (SEC)  is based on the discrimination of individual sample components by the pores of the packing material. It is the dominant mode of separation for polymers. Large sample molecules cannot or can only partially penetrate the pores, whereas smaller molecules can access all or a larger number of pores.

In SEC, large molecules elute from the column first, smaller molecules elute later, and the smallest molecules that can access all the pores elute last from the column. Size exclusion chromatography is the only mode of chromatography that does not involve interaction with a stationary phase by means of adsorption or partitioning of the solutes.

Particles prepared from organic polymers have traditionally been the most widely used packing materials for size-exclusion chromatography (SEC). The terms SEC, GFC (gel filtration chromatography) and GPC (gel permeation chromatography) all refer to the same chromatographic technique. In GFC an aqueous mobile phase is used, while an organic mobile phase is employed in GPC. The general term SEC covers both uses.

GFC is popular among biochemists for the isolation of protein fractions or for the removal of aggregates in a final polishing step in biotechnology production. GFC is also frequently used for desalting a (protein) sample solution, often to prepare the sample for elution by another chromatographic mode.

GPC plays an important role in the characterization of industrial organic-soluble polymers.

Tosoh Bioscience LLC 3604 Horizon Drive, Suite 100, King of Prussia, PA, 19406 Tel: (800) 366-4875  Email: info.tbl@tosoh.com