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Tosoh Bioscience LLC: The Chemistry of Innovation
Size Exclusion: Gel Permeation Chromatography
Proteins
Water-soluble Polymers
Polar Organic-soluble Polymers
Alpha
SuperAW
Organic-soluble Polymers


TSKgel Alpha and SuperAW columns were developed for the GPC analysis of polymers of intermediate polarity. As in the TSKgel PW- and PWxl-type resins, the particles in TSKgel Alpha and SuperAW columns have a hydroxylated methacrylate polymer backbone, but they are crosslinked to a higher degree than TSKgel PW- and PWxl-type columns to minimize swelling in polar organic solvents.

TSKgel Alpha and SuperAW particles are available in a wide variety of pore sizes.

- Use semi-micro TSKgel SuperAW columns

  • for high-throughput applications
  • to reduce solvent consumption
  • to reduce solvent disposal cost.

- Use TSKgel Alpha columns

  • when analysis time is not critical
  • when sample mass is not limited
  • to collect fractions
  • to obtain maximum number of plates (at the expense of analyis time).

Highlights:

  • TSKgel Alpha and SuperAW columns
    • Particle sizes
      • TSKgel SuperAW, 4-9μm particles
      • TSKgel Alpha, 7-13μm particles
    • No or minimal swelling in a wide range of polar organic solvents: methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP.
    • For high thoughput applications, TSKgel SuperAW columns provide equivalent resolution in 1/2 the time compared to TSKgel Alpha columns.

    • Accurate MW determination and normal retention of polystrene polymers in DMF solvent.

    • System peaks from salts in the eluent elute away from the oligomer of interest.

  • Applications: polymers that are soluble in methanol, acetonitrile, DMSO, isopropanol, or THF; can also be used for water-soluble polymers.


TSKgel SuperAW

TSKgel Alpha

TSKgel SuperAW column line consists of five columns and a mixed-bed column.

High efficiency TSKgel SuperAW columns are only available in 6.0mm ID x 15cm columns for High Throughput applications.

TSKgel Alpha column line consist of five columns and a mixed-bed column.

TSKgel Alpha columns are available in conventional 7.8mm ID x 30cm column formats.

 


Contact us:

Tech Service:
800-366-4875 (option #3)

Customer Service:
866-527-3587

info.tbl@tosoh.com
 


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Application Overview -- Gel Filtration Chromatography with TSKgel columns

Tosoh Bioscience LLC 3604 Horizon Drive, Suite 100, King of Prussia, PA, 19406 Tel: (800) 366-4875  Email: info.tbl@tosoh.com