TSKgel SW-type columns are silica-based. A hydrophilic diol-type bonded phase shields the silica surface from interacting with protein samples. Three different pore sizes are available. 125Å pore size for analysis of small proteins and peptides 250Å pore size for most protein samples 450Å pore size for very large proteins and nucleic acids Compared to competitive HPLC columns, SW-type columns contain more pore volume per unit column volume, which results in higher MW selectivity or better resolution. Particle sizes for the three SW-type and the BioAssist DS column lines are: SuperSW: 4 micron SWxl: 5-8 micron SW: 10-13 micron BioAssist DS: 15 micron See each of the SW-type and the BioAssist DS product lines for representative calibration curves.

Highlights TSKgel SW-type packings are comprised of rigid spherical silica gel particles chemically derivatized with diol-containing ligands. Low adsorption and well-defined pore size distribution, which are requirements for high performance Gel Filtration of proteins. Three pore sizes are available in TSKgel SW or SWxl product lines, two in the TSKgel SuperSW product line, and one in the TSKgel BioAssist DS product line.  TSKgel SuperSW columns feature 4 micron particles for the highest resolution. The narrow 4.6mm column ID provides the highest sensitivity when limited in sample mass. Semi-preparative (21.5mm ID)  stainless steel columns are available for precise scale-up to commercial production. PEEK and Glass column hardware are available in the TSKgel SWxl product line. PEEK hardware is available in TSKgel BioAssist DS product line.

TSKgel  SuperSW	TSKgel SWxl	TSKgel SW	TSKgel BioAssist DS
TSKgel SuperSW columns contain spherical 4µm particles (125Å and 250Å only) packed in 30cm x 4.6mm ID columns for: For R&D. Use to benefit from high sensitivity when limited in sample mass 2.6-fold reduction in solvent consumption Use 5 or 8µm particle size TSKgel SWxl columns to improve sample resolution or to reduce analysis time (over SW columns). For QC of mAb Select 15cm x 7.8mm ID columns for high speed analysis Use PEEK columns to reduce sample adsorption to stainless steel or glass Analytical TSKgel SW column line features 10 or 13µm particle sizes for improved sample capacity. For protein isolation. Select from stainless steel or glass column hardware. Use preparative columns up to 55mm ID for larger scale purifications. TSKgel BioAssist DS material packed with 15µm particle sizes. For desalting and buffer exchange of protein and nucleic acid samples Remove detergents and denaturants Select from 4.6mm or  10mm ID x 15cm PEEK column hardware.

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Application Overview -- Gel Filtration Chromatography with TSKgel columns