TSKgel BioAssist DS columns are designed for the desalting and buffer exchange of proteins and polynucleotides at semi-preparative scale. Packed with 15μm polyacrylamide beads in PEEK hardware, TSKgel BioAssist DS columns show excellent desalting performance.

The novel* hydrophilic highly cross-linked polyacrylamide beads exhibit superior mechanical strength compared with conventional hydrophilic polyacrylamide beads and cross-linked dextran beads. This increase in strength is what allows the use of the small spherical 15μm beads. TSKgel BioAssist DS columns can be operated in standard HPLC systems to quickly and efficiently reduce salt and/or buffer concentrations of collected protein or nucleic acid fractions.

*US patent number 7,659,348

Product Attributes:

Base material:   Urea cross-linked polyacrylamide  Particle size: 15μm  Pore size: Excludes 2500Da PEG  Particle porosity: ca. 60%  Mechanical strength: <4MPa

Literature:

Application Note -- A Novel Desalting Column With High Mechanical Strength for Faster Desalting of Proteins

Catalog -- SEC-SW Series Columns

Operating Conditions and Specifications 

Technical Presentation -- Characteristics of a Desalting Column for Biomolecular Analysis: the TSKgel BioAssist DS Column

Technical Presentation -- A Novel Desalting Column with High Mechanical Strength for Faster Desalting of Proteins

Technical Presentation -- Fast Desalting of Proteins Using a Novel High Mechanical Strength Gel Filtration Column