SW

TSKgel SW columns, introduced in 1977, were the first of a long line of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristics
TSKgel SW-type columns are based on highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SW-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes.

Calibration Curves
The figure below shows the calibration curves for globular proteins, polyethylene oxides and dextrans for each of the three TSKgel SW-type columns. The following table summarizes the molecular weight ranges for TSKgel G2000SW, G3000SW and G4000SW columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the particle pores. For comparative purposes, similar data is provided as well for TSKgel SWxl and SuperSW-type columns.

Polyethylene oxide, dextran and protein calibration curves for TSKgel SW columns

Properties and separation ranges for TSKgel SW-type packings (Molecular Weight in Da)

TSKgel packing	Particle size (µm)	Pore size (Å)	Globular proteins	Dextrans	Polyethylene glycols and oxides
SuperSW2000	4	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
G2000SWxl/ BioAssist G2SWxl	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
QC-PAK TSK200	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
G2000SW	10, 13	125	5,000 - 1 x 10^5	1,000 - 3 x 10^4	500 - 15,000

SuperSW3000	4	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
G3000SWxl/ BioAssist G3SWxl	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
QC-PAK TSK300	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
G3000SW	10	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4

G4000SWxl/ BioAssist G4SWxl	8	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5
G4000SW	13, 17	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5

Data generated using the following conditions:

Columns:	Two 4µm, 4.6mm ID x 30cm TSKgel SuperSW columns in series; two 5µm, 7.8mm ID x 30cm TSKgel SWxl columns in series; two 10µm, 7.5mm ID x 60cm TSKgel SW columns in series
Elution:	Globular proteins: 0.3M NaCl in 0.1M (0.05M for TSKgel SWxl columns) phosphate buffer, pH 7 Dextrans and polyethylene glycols and oxides (PEOs): distilled water

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Application Overview -- Gel Filtration Chromatography with TSKgel columns

Tosoh Bioscience LLC 3604 Horizon Drive, Suite 100, King of Prussia, PA, 19406 Tel: (800) 366-4875 Email: info.tbl@tosoh.com