TSKgel SWxl columns, introduced in 1987, represent the second generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristics
TSKgel SWxl-type columns contain smaller particles than TSKgel SW-type columns; 5 and 8μm versus 10 and 13μm. As the TSKgel SW-type columns, TSKgel SWxl columns feature highly porous silica particles, the surface of which has been shielded from interacting with proteins by derivatization with ligands containing diol functional groups. TSKgel SWxl-type columns stand out from other silica- or polymer-based high performance size exclusion columns by virtue of their large pore volumes. These columns are commonly used in the quality control of monoclonal antibodies and other biopharma products.

Properties and separation ranges for TSKgel SW-type packings (Molecular Weight in Da)

TSKgel   packing Particle size (µm) Pore size (Å) Globular proteins Dextrans Polyethylene glycols and oxides SuperSW2000 4 125 5,000 - 1.5 x 10^5 1,000 - 3 x 10^4 500 - 15,000 G2000SWxl BioAssist G2SWxl 5 125 5,000 - 1.5 x 10^5 1,000 - 3 x 10^4 500 - 15,000 QC-PAK TSK200 5 125 5,000 - 1.5 x 10^5 1,000 - 3 x 10^4 500 - 15,000 G2000SW 10, 13 125 5,000 - 1 x 10^5 1,000 - 3 x 10^4 500 - 15,000 SuperSW3000 4 250 1 x 10^4 - 5 x 10^5 2,000 - 7 x 10^4 1,000 - 3.5 x 10^4 G3000SWxl BioAssist G3SWxl 5 250 1 x 10^4 - 5 x 10^5 2,000 - 7 x 10^4 1,000 - 3.5 x 10^4 QC-PAK TSK300 5 250 1 x 10^4 - 5 x 10^5 2,000 - 7 x 10^4 1,000 - 3.5 x 10^4 G3000SW 10 250 1 x 10^4 - 5 x 10^5 2,000 - 7 x 10^4 1,000 - 3.5 x 10^4             G4000SWxl BioAssist G4SWxl 8 450 2 x 10^4 - 7 x 10^6 4,000 - 5 x 10^5 2,000 - 2.5 x 10^5 G4000SW 13, 17 450 2 x 10^4 - 7 x 10^6 4,000 - 5 x 10^5 2,000 - 2.5 x 10^5

Data generated using the following conditions:

Columns:	Two 4µm, 4.6mm ID x 30cm TSKgel SuperSW columns in series; two 5µm, 7.8mm ID x 30cm TSKgel SWxl columns in series; two 10µm, 7.5mm ID x 60cm TSKgelSW columns in series
Elution:	Globular proteins: 0.3M NaCl in 0.1M (0.05M for TSKgel SWxl columns) phosphate buffer, pH 7 Dextrans and polyethylene glycols and oxides (PEOs): distilled water

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Application Overview -- Gel Filtration Chromatography with TSKgel columns