SuperSW

TSKgel SuperSW columns, introduced in 1997, represent the third generation of high performance Gel Filtration columns that have become synonymous with analyzing protein molecular weights in the emerging field of biotechnology.

Particle Characteristics
TSKgel SuperSW-type columns contain smaller particles than TSKgel SWxl-type columns; 4 micron versus 5 micron. In addition, the column internal diameter has been reduced from 7.8mm ID to 4.6mm ID to provide higher sensitivity in sample-limited cases and to cut down on solvent use.

HPLC System Requirements
It is important to employ an HPLC system that is optimized with regards to extra-column bandbroadening to take full advantage of the high column efficiency that can be obtained on TSKgel SuperSW columns. See Separation Report 95 for recommendations.

Calibration Curves
The figure to the right shows the calibration curves for globular proteins for the two TSKgel SuperSW-type columns in comparison with those for the corresponding TSKgel SWxl columns. The following table summarizes the molecular weight ranges for TSKgel SuperSW2000 and SuperSW3000 columns for globular proteins, polyethylene oxides and dextrans that can, at least partially, access the pores. For comparative purposes, similar data is provided as well for TSKgel SW and SWxl-type columns.

Protein Calibration curves for
TSKgel SuperSW and SWxl

Properties and separation ranges for TSKgel SW-type packings (Molecular Weight in Da)

TSKgel packing	Particle size (µm)	Pore size (Å)	Globular proteins	Dextrans	Polyethylene glycols and oxides
SuperSW2000	4	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
G2000SWxl BioAssist G2SWxl	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
QC-PAK TSK200	5	125	5,000 - 1.5 x 10^5	1,000 - 3 x 10^4	500 - 15,000
G2000SW	10, 13	125	5,000 - 1 x 10^5	1,000 - 3 x 10^4	500 - 15,000

SuperSW3000	4	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
G3000SWxl BioAssist G3SWxl	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
QC-PAK TSK300	5	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4
G3000SW	10	250	1 x 10^4 - 5 x 10^5	2,000 - 7 x 10^4	1,000 - 3.5 x 10^4

G4000SWxl BioAssist G4SWxl	8	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5
G4000SW	13, 17	450	2 x 10^4 - 7 x 10^6	4,000 - 5 x 10^5	2,000 - 2.5 x 10^5

Data in the Table were generated using the following conditions:

Columns:	Two 4µm, 4.6mm ID x 30cm TSKgel SuperSW columns in series; two 5µm, 7.8mm ID x 30cm TSKgel SWxl columns in series; two 10µm, 7.5mm ID x 60cm TSKgel SW columns in series
Elution:	Globular proteins: 0.3M NaCl in 0.1M (0.05M for TSKgel SWxl columns) phosphate buffer, pH 7 Dextrans and polyethylene glycols and oxides (PEOs): distilled water

Contact us:

Tech Service:
800-366-4875 (option #3)

Customer Service:
866-527-3587

info.tbl@tosoh.com

Return to HPLC Columns main page

Return to Size Exclusion main page

Application Overview -- Gel Filtration Chromatography with TSKgel columns

Tosoh Bioscience LLC 3604 Horizon Drive, Suite 100, King of Prussia, PA, 19406 Tel: (800) 366-4875 Email: info.tbl@tosoh.com