TSKgel PWxl columns are composed of spherical, hydrophilic polymethacrylate beads. The main application area for TSKgel PWxl columns is the analysis of water-soluble polymers, such as celluloses, acrylamides, glycols, dextrans, polyvinylalcohol, and oligosaccharides. See the table on the TSKgel PW page for typical samples and suitable eluents. Figure 1 shows the calibration curves for all TSKgel PWxl-type columns. Figure 2 shows the benefits of TSKgel PWxl columns over TSKgel PW columns, namely the improved resolution due to the smaller particle sizes used in TSKgel PWxl columns.

Figure 1: Polyethylene glycol and oxide calibration curves on TSKgel PWxl columns Figure 2: Faster analysis and higher resolution with TSKgel PWxl columns

Although proteins can be analyzed on TSKgel PWxl columns, pore volumes are much smaller than on TSKgel SW-type columns. Figure 3 demonstrates the limited separation power of TSKgel PWxl for protein samples. Specialty Columns TSKgel G-DNA-PW is available for the analysis of DNA fragments. Similarly, TSKgel G-Oligo-PW is the preferred column for the analysis of oligosaccharides. The mixed-mode column, TSKgel GMPWxl, has an extended linear calibration range, suitable for samples with a broad MW distribution and unknowns. A smaller particle size version of the popular TSKgel G-Oligo-PW column for oligomer separation is now also available. By using  3 micron instead of 7 micron particles a separation performed on an TSKgel  G-Oligo-PW column can be done in half the time on a TSKgel SuperOligoPW column.

Figure 3: Protein Calibration curves for TSKgel PWxl

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Application Overview -- Gel Filtration Chromatography with TSKgel columns

Separation Report -- Introduction of Aqueous SEC Columns: TSKgel PWxl series

Use TSKgel SuperOligoPW columns for the determination of molecular mass of water-soluble oligomers and low MW polymers.