Besides determining of “what sticks” during resin screening experiments, ToyoScreen columns can be used to quickly establish optimum elution conditions.   Varying pH, salt type, salt gradients and flow rate are common experimental parameters explored.  The effect of varying salt type and pH are shown in the figures below for Anti-TSH in cell culture supernatant on ToyoScreen Phenyl-650M. 

Optimizing eluent pH in HIC	Optimizing salt conditions in HIC