HIC

Toyopearl hydrophobic interaction Chromatography media (HIC) are available in six different chemistries ranging in hydrophobicity from Ether-650 (low) to Hexyl-650 (high).  Depending on the feedstock and the impurity profile, the determination of the best selectivity is largely an empirical process.  The figures below show the selectivity differences of the ToyoScreen HIC Mix Pack chemistries on the separation of protein standards and Anti-IgG from albumin in mouse ascites fluid. 

HIC resins - Protein Standards	HIC resins - Mouse Ascites Fluid (Anti-IgE)

 

IEC

Ion exchange chromatography (IEC) separates molecules based on the ionic interaction of the molecule with the charged support. The individual functional group and its pKa can be used to evaluate different selectivities in chromatographic separations. ToyoScreen columns are offered in both strong and weak functionalities for both cation and anion ligand types. The figures below show the effect on the separation for the available Toyopearl ion exchange chemistries when screening protein standards.

Anion Exchange Resins - Protein Standards	Cation Exchange Resins - Protein Standards

 

AFC

In affinity chromatography (AFC), the ligands employed are specific to a particular protein class or functional group on the accessible surface of the target molecule.  ToyoScreen affinity columns are offered in three group specific ligand chemistries: AF-Blue HC-650M, AF-Chelate 650M, and AF-Red 650M. AF-Blue 650M is specific for kinases, phosphatases, dehydrogenases and other molecules such as albumin and blood coagulation factors.  AF-Red-650M is specific for dehydrogenases and other proteins such as plasminogen.  AF-Chelate is an excellent resin for metal ligand affinity for molecules containing His-tags. ToyoScreen affinity columns allow for the quick assessment of optimum binding conditions for any of these columns.