Toyopearl AF-rProtein A-650F utilizes a new alkaline resistant recombinant Protein A variant. This new non-animal derived ligand is bound to the base bead, Toyopearl HW-65F, using multipoint attachment. This contributes to both the new resin's alkaline resistance and its low ligand leakages in the range 5-25ng/mg IgG.
Toyopearl AF-rProtein A-650F maintains its capacity well after repeated CIP exposure to 0.1mol/L NaOH (figure 1) and has reasonable tolerance to 0.5mol/L NaOH CIP (figure 2).
The new resin also tolerates exposure to above ambient temperatures (figure 3) and if needed can be autoclaved.
Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.
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