TSKgel Ether-5PW is the highest hydrophilicity of the five HIC ligands offered by TBL. It is useful for the separation of very hydrophobic molecules such as membrane proteins.  It is typically used for high performance chromatography and polishing steps. It has the same selectivity as the larger particle Toyopearl Ether-650 products. It often is used instead of cross-linked dextrans with exclusion limits of 10,000 MW.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Pore size (mean):  1,000Å  Particle size (mean):  20µm   30µm  Pressure rating:  20 bar  Shipping buffer:  20% ethanol  pH stability:  1-13  Shelf life:  10 years

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography