Toyopearl Hexyl-650 has the highest hydrophobicity of the HIC ligands offered by TBL. It is used for very hydrophilic proteins or where a low salt elution environment is required. It is useful as an early capture step or for intermediate purification.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Pore size (mean):  1,000Å  Particle size (mean):  100µm (C-grade)   Pressure rating:  3 bar  Shipping buffer:  20% ethanol  pH stability:  1-13  Shelf life:  10 years

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography