Toyopearl Phenyl-600 has the middle hydrophobicity of the five HIC ligands offered by TBL. Its pore size has been optimized for the chromatography of monoclonal antibodies and it is typically used in an intermediate or polishing step.

It is a very high recovery and high dynamic capacity resin for both large and small proteins. When used as the polishing step up to an extra 5% yield may be seen compared to other Phenyl resins.

To see other products optimized for HIC purification of mAbs please see Toyopearl PPG-600 and Toyopearl Butyl-600.

Structure:

Hydrophobicity:

Ether < PPG < Phenyl < Butyl < Hexyl
(least to most)

Product Attributes:

Pore size (mean):  750Å  Particle size (mean):  65µm (M-grade)  Pressure rating:  3 bar  Shipping buffer:  20% ethanol  pH stability:  1-13  Shelf life:  10 years

Literature:

Catalog - Process Media, Hydrophobic Interaction Chromatography