Toyopearl GigaCap S-650M resin has excellent base stability in 1.0mol/L NaOH (see Figure below and Table 1) and shows robust clean in place properties with minimal loss of capacity after 50 X 1 hour cycles of caustic exposure using 1.0mol/L NaOH.
Alkaline stability
Table 1 - Toyopearl GigaCap S-650M resin is stable in 1mol/L NaOH
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|
Start |
|
Week 3 |
Week 5 |
|
IEC capacity, meq/mL |
0.159 |
|
0.158 |
0.156 |
|
hIgG DBC*, mg/mL gel |
143 |
144 |
140 |
135 |
|
hIgG recovery (%) |
99 |
101 |
100 |
99 | |
Conditions: resin was soaked in 1mol/L NaOh for 5 weeks.
*Dynamic Binding Capacity measured at 10% breakthrough
Clean in place stability
Column exposed to 50 cycles of 1.0mol/L NaOH at 280cm/hr and the dynamic binding capacity of lysozyme was determined (exposure time at least 1 hr per cycle)
Table 2 - DBC of lysozyme after 50 CIP cycles with 0.1mol/L NaOH
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|
Prior to NaOH |
|
|
Toyopearl GigaCap S-650M |
167mg/mL resin |
| |
|