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Tosoh Bioscience LLC: The Chemistry of Innovation
Process Media Products
Process Media Products
Ion Exchange (IEC)
Hydrophobic Interaction (HIC)
Hydroxyapatite (HA)
Protein A
Toyopearl AF-rProtein A HC-650
Toyopearl AF-rProtein A-650
Affinity (AFC)
Size Exclusion (SEC)

is a high capacity Protein A resin for monoclonal antibody purification. An enhanced rProtein A ligand is bound to the TOYOPEARL HW-65F base bead via multipoint attachment resulting in excellent base stability for up to 200 CIP cycles with 0.1 mol/L NaOH.

TOYOPEARL AF-rProtein A HC-650F resin maintains 80% of initial dynamic binding capacity after 40 CIP cycles with 0.5 mol/L NaOH. TOYOPEARL AF-rProtein A HC-650F resin exhibits dynamic binding capacities of greater than 65 g/L at residence times of 5 minutes and greater than 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L.

Achievement of high linear velocities at relatively low pressure enables high throughput at production scale using equipment with moderate pressure limitations. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose base stable resins. Typical leakage for this rProtein A ligand is 0.6 -1.7 ng rProtein A /mg eluted antibody by ELISA testing. Though TOYOPEARL AF-rProtein A-650F resin is shipped in 20% ethanol, it can be stored in 2% benzyl alcohol if necessary.

Good pressure-flow characteristics and kinetics contribute to maintaining good capacity particularly at low residence times.



Product Attributes:

 Pore size (mean):  100 nm
 Particle size (mean):  45 µm (F-grade) 
 Pressure rating:  0.3 MPa
 Shipping buffer:  20% ethanol
 pH stability:  2-13

 Shelf life
(room temperature):

1 year (ongoing)


Application Note -- Monoclonal Antibody Purification with a High Capacity Protein A Resin

Application Note -- TOYOPEARL AF-rProtein A HC-650F Host Cell Protein Removal

Application Note -- TOYOPEARL AF-rProtein A HC-650F rProtein A Ligand Leakage

Application Note -- Purification of a Monoclonal Antibody with TOYOPEARL AF-rProtein A HC-650F at Various Bed Heights

Application Note -- Comparison of TOYOPEARL AF-rProtein A HC-650F Binding Capacity at Various Bed Heights and Constant Linear Velocity

Catalog - Process Media, Protein A Chromatography

Operating Conditions and Specifications

Product Overview -- TOYOPEARL AF-rProtein A HC-650F

Technical Presentation -- Characterization of a Newly Developed High Capacity, Alkaline Resistant, Recombinant Protein A Resin

Technical Presentation -- Comparison of Binding Capacity of TOYOPEARL AF-rProtein A HC-650F Affinity Resin at Varying Bed Heights and Constant Column Volume


Toyopearl rProtein A-650 data

DBC of of TOYOPEARL AF-rProtein A HC-650F resin with various feedstock titers

Base stability of TOYOPEARL AF-rProtein A HC-650F


DBC of TOYOPEARL AF-rProtein A HC-650F resin after CIP with 0.5 mol/L NaOH


Ordering information:
To place an order please click on the shopping cart next to the desired item, or contact Customer Service. Tosoh Bioscience LLC only accepts orders from customers in North and South America. For sales offices in other parts of the world, please click Locations.


DescriptionResin volumeParticle sizePrice ($)


TOYOPEARL AF-rProtein A HC-650F10 mL45 µm799Add to Cart


TOYOPEARL AF-rProtein A HC-650F25 mL45 µm1,377Add to Cart


TOYOPEARL AF-rProtein A HC-650F100 mL45 µm2,754Add to Cart


TOYOPEARL AF-rProtein A HC-650F1 L45 µmInquire


TOYOPEARL AF-rProtein A HC-650F5 L45 µmInquire


TOYOPEARL AF-rProtein A HC-650F50 L45 µmInquire


ToyoScreen AF-rProtein A HC-650F1 mL x 5 ea.45 µm690Add to Cart


ToyoScreen AF-rProtein A HC-650F5 mL x 1 ea.45 µm637Add to Cart


ToyoScreen AF-rProtein A HC-650F5 mL x 5 ea.45 µm2,070Add to Cart


MiniChrom TOYOPEARL AF-rProtein A HC-650F5 mL45 µm818Add to Cart


ELISA Kit for TOYOPEARL AF-rProtein A HC-650F*892Add to Cart
*This kit is specifically prepared for Toyopearl AF-rProtein A HC-650F. Test kits for other commercially available Protein A products may not work properly for Toyopearl AF-rProtein A HC-650F.

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Tech Service:
800-366-4875 (option #3)

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View this archived webinar:

Purification of Monoclonal Antibodies with High Capacity Protein A Resins: What are the Benefits?

Main Process Page

Tosoh Bioscience LLC 3604 Horizon Drive, Suite 100, King of Prussia, PA, 19406 Tel: (800) 366-4875  Email: info.tbl@tosoh.com