Unlike other TSK-GEL affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups.  The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.

 Applications include: glycoproteins, antigens, etc.

Structure:

Product Attributes:

Pore size (mean): 1000Å  Particle size (mean): 10µm  pH stability: 2.0 - 12.0  Ligand conc.: ca. 20µmol/mL resin  Adsorption capacity: > 60mg/g dry resin (coupling capacity w/soybean trypsin inhibitor)  Exclusion limit  (base resin, estimate): < 10,000,000 Da globular proteins  Active group:  tresyl

Literature:

Catalog -- Chromatography Catalog, AFC Columns

Operating Conditions and Specifications 

Separation Report -- Separation of Monoclonal Antibodies Using TSK-GEL HPLC Columns

Brochure -- TSK-GEL HPLC Columns for Biomolecular Applications

Ordering information:
To place an order please click on the shopping cart next to the desired item, or contact Customer Service. Tosoh Bioscience LLC only accepts orders from customers in North and South America. For sales offices in other parts of the world, please click Locations.
 

P/N Description Particle size Housing material ID (mm) Length (cm)   14455 TSKgel Tresyl-5PW 10μm Stainless Steel 6 4 14456 TSKgel Tresyl-5PW 10μm Stainless Steel 7.5 7.5 16208 TSKgel Tresyl-5PW Guardgel, 2g 10μm