1. What is the support material for Tosoh Bioscience affinity resins? (back to top)

TSKgel affinity chromatography columns are based upon the G5000PW methacrylate porous resin. This “5PW” media is hydrophilic, with 1000 Angstrom pores and an estimated protein size exclusion limit of 1000 kilodaltons.

2. What are the ligand concentrations, adsorption capacities and test analytes for the various Affinity column supplied by Tosoh Bioscience? (back to top)

Characteristics of TSKgel AFC columns

3. Can I get a TSKgel Affinity LC column with non-metal wetted parts? (back to top)

Yes. We supply glass columns in two formats: 5mm ID x 5cm and 8mm ID x 7.5cm with 10µm pore size Pyrex frits. 

The shipping solvents for these columns are as follows:

Boronate-5PW:                             distilled water
Chelate-5PW:                               acetate buffer
Tresyl-5PW:                                 acetone
Heparin-5PW                                distilled water

4. How do I load and desorb protein from a TSKgel affinity column? (back to top)

Specific instructions for retaining your protein as well as desorption procedures are included with each affinity column. Keep in the mind the analyte requires some structural feature that coincides with the retention mechanism of the affinity resin.

5. What endfitting do I use with my particular TSKgel Affinity column? (back to top)  

Please visit the HPLC Columns - Replacement Parts page, which will provide a description of the available fittings, including the size of the frit in the fitting and the applicable endfitting to be used with your TSKgel column.  Question #10 within the General FAQ category includes additional information on fittings and connectors.

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