1. What is the difference between TSK-GEL Super-ODS and ODS-140HTP columns?
TSK-GEL Super-ODS reversed phase columns, when introduced in 1995, were the first commercially available columns packed with sub-3 micron particles. Often referred to as 2 micron packed columns, the mean particle size of the silica is 2.3 micron. The same silica lots used to prepare TSK-GEL Super-ODS columns are used in the manufacture of TSK-GEL ODS-140HTP columns.
There are two important differences between the two column types. First, although the same polymeric C18 bonding chemistry is used to produce TSK-GEL Super-ODS and ODS-140HTP columns, the endcapping reaction on TSK-GEL ODS-140HTP columns is more exhaustive than on TSK-GEL Super-ODS columns (please see the following paragraph for additional details). In addition, TSK-GEL Super-ODS (as well as Super-Octyl and Super-Phenyl columns) have restrictions for flow rate as well as pressure. For instance, the 4.6mm ID TSK-GEL Super-ODS columns cannot be operated above 30MPa, nor above 4mL/min. In contrast, the upper pressure limit for 2.1mm ID TSK-GEL ODS-140HTP columns is twice as high at 60MPa, and there is no upper limit for the flow rate, making TSK-GEL ODS-140HTP columns your TSK-GEL columns of choice for use at ultra-high pressures and flow rates.
The more exhaustive endcapping reaction that is performed on TSK-GEL ODS-140HTP columns means (in order of importance): 1. shorter retention, higher efficiency and better peak shape for basic compounds 2. slightly shorter retention of polar compounds 3. improved chromatography of chelating compounds.
2. What are the naming conventions for Tosoh Bioscience RPLC columns?
The TSK-GEL designation is the company’s trademark for all liquid chromatography columns. The letters “ODS” stand for octadecylsilane or C18 bonded to a silica support material. The number 80 or 120 in the name denotes the pore size of the resin in Angstrom. For instance, the silica in TSK-GEL ODS-140HTP columns has a pore size of 140 Angstrom. The letters Tm or Ts refer to an endcapped column (minimal free silanol groups). The endcapping in Ts is more exhaustive than that found in the Tm products. Tosoh Bioscience also supplies octyl (C8), phenyl, cyano (CN) and trimethylsilane (C1) columns for RPLC.
Example: TSKgel ODS-80Tm = C18 on silica with 80 angstrom pores, endcapped.
3. How do I select the optimal RP column for my application?
The choice of a RPLC column is based upon the solubility and polarity of the test analytes. The more hydrophobic the sample molecule the less hydrophobic the solid support must be. Conversely, the less hydrophobic the sample molecule the more hydrophobic the solid support. For example, protein digests and low MW pharmaceuticals, waste water contaminants, etc. will generally be suited for a C18 column. More hydrophobic molecules with extensive aromatic nature may be best suited to C8 or phenyl ligands.
4. Do you have a Guide to Column Selection?
Column Selection for TSK-GEL Reversed Phase Columns
5. What are “Super Series” RPLC Columns?
Super Series RPLC columns are monodispersed spherical silica particles with 2mm particle diameter and 100 Angstrom pores. They are available with ODS, Octyl and Phenyl ligands and cover a full range of polarities (hydrophobicities). An exhaustive endcapping process assures minimal silanol activity. The small particle size resin used results in very high separation efficiency, good resolution and increased speed of analysis.
6. Does Tosoh Bioscience also offer polymeric based RPLC columns?
Yes. Polymer based RPLC columns are stable in a pH range of 2-12. Samples can be run at high pH without any deterioration of the column plus the column can be cleaned with strong acids and bases. Tosoh Bioscience supplies these columns with non-porous resins (NPR) as well a choice of pore sizes. Column selection is dictated by the sample molecular weight and its polarity in solution.
7. What are chemical and physical properties of TSK-GEL Reversed Phase LC columns?
Properties of silica-based TSK-GEL RPC columns
Properties of polymer-based TSK-GEL RPC columns
8. What endfitting do I use with my particular TSKgel Reversed Phase column?
Please visit the HPLC Columns - Replacement Parts page, which will provide a description of the available fittings, including the size of the frit in the fitting and the applicable endfitting to be used with your TSKgel column. Question #4 within the General FAQ category includes additional information on fittings and connectors.