1. What are the naming conventions for Tosoh Bioscience RPLC columns?
The TSK-GEL designation is the company’s trademark for all liquid chromatography columns. The letters “ODS” stand for octadecylsilane or C18 bonded to a silica support material. The number 80 or 120 in the name denotes the pore size of the resin in Angstroms. The letters Tm or Ts refer to an endcapped column (minimal free silanol groups). The endcapping in Ts is more exhaustive than that found in the Tm products. Tosoh Bioscience also supplies octyl (C8), phenyl, cyano (CN) and trimethylsilane (C1) columns for RPLC.
Example: TSKgel ODS-80Tm = C18 on silica with 80 angstrom pores, endcapped.
2. How do I select the optimal RP column for my application?
The choice of a RPLC column is based upon the solubility and polarity of the test analytes. The more hydrophobic the sample molecule the less hydrophobic the solid support must be. Conversely, the less hydrophobic the sample molecule the more hydrophobic the solid support. For example, protein digests and low MW pharmaceuticals, waste water contaminants, etc. will generally be suited for a C18 column. More hydrophobic molecules with extensive aromatic nature may be best suited to C8 or phenyl ligands.
3. Do you have a Guide to Column Selection?
Column Selection for TSK-GEL Reversed Phase Columns
4. What are “Super Series” RPLC Columns?
Super Series RPLC columns are monodispersed spherical silica particles with 2mm particle diameter and 100 Angstrom pores. They are available with ODS, Octyl and Phenyl ligands and cover a full range of polarities (hydrophobicities). An exhaustive endcapping process assures minimal silanol activity. The small particle size resin used results in very high separation efficiency, good resolution and increased speed of analysis.
5. Does Tosoh Bioscience also offer polymeric based RPLC columns?
Yes. Polymer based RPLC columns are stable in a pH range of 2-12. Samples can be run at high pH without any deterioration of the column plus the column can be cleaned with strong acids and bases. Tosoh Bioscience supplies these columns with non-porous resins (NPR) as well a choice of pore sizes. Column selection is dictated by the sample molecular weight and its polarity in solution.
6. What are chemical and physical properties of TSK-GEL Reversed Phase LC columns?
Properties of silica-based TSK-GEL RPC columns
Properties of polymer-based TSK-GEL RPC columns
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USP Reference for TSK-GEL columns |