1. Where do I find the coupling procedure for the activated resins?

Contact the Technical Support line at 800-366-4875 and a technical specialist can provide a detailed procedure for the activated resins. The Tosoh Bioscience Literature Library also has many recommended prodcedures based on the type of reactive or activated resin being used.

2. Can you clean affinity resins with caustic?

Toyopearl AF-Chelate-650M and, in extreme cases where other reagents do not remove residual protein, Toyopearl AF-Heparin HC-650M, can be cleaned with caustic up to 0.5M NaOH.  All other Affinity products such as the activated media are most likely sensitive to caustic (due to the nature of the attached ligand) and should be cleaned with less aggressive combinations of salt, chaotrope, & detergent.

3. Does Tosoh Bioscience offer a Protein A resin for antibody purification?

Tosoh Bioscience does not manufacture a Protein A resin for antibody purification. Data has been collected to support the use of Toyopearl Ion Exchange and Hydrophobic Interaction resins for downstream purification after a Protein A capture step for antibody purification.

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