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Journal of Chromatography A: Development of a comprehensive two-dimensional hydrophilic interaction chromatography/quadrupole time-of-flight mass spectrometry system and its application in separation and identification of saponins from Quillaja saponaria
Journal of Chromatography A, Volume 1181, Issues 1-2, 15 February 2008, Pages 51-59 Yuan Wang, Xin Lu, Guowang Xu
Hydrophilic interaction chromatography (HILIC) was used in two dimensions in a comprehensive two-dimensional HILIC hyphenated with a quadrupole time-of-flight mass spectrometry (HILIC × HILIC-Q-TOF-MS) system for the analysis of complex samples of hydrophilic compounds. A TSKgel Amide-80 column was employed as the first dimension…
Separation Reports
SR105: Semi-micro Columns for Size Exclusion Chromatography in Organic Solvent Systems: TSK-GEL SuperMultiporeHZ Series Columns
SEC is a method for calculating molecular weight from a calibration curve created using molecular weight standards. However, challenges arise when SEC is used to analyze polymers with a wide molar mass distribution, namely distortion of the chromatogram or deviations between the actual calibration curve and the calibration curve approximated from data obtained from the molecular weight standards. TSK-GEL SupermultiporeHZ Series SEC columns contain particles that are mono-disperse in size and exhibit a broad range of pore sizes. This multi-pore particle synthesis technology, developed by Tosoh scientists, ensures that each particle has an extended linear calibration curve, thereby greatly diminishing chromatograms with inflection points.
SR094: Analyzing Polymers that are More Soluble in Polar-Organic Solvents with TSK-GEL Alpha Series Columns
Size-exclusion columns are usually classified into the following types: columns for aqueous solvent systems and columns used with an organic solvent systems. However, multiple problems have occurred with each of these types of columns when used to study polar polymer molecules. With the use of TSK-GEL Alpha Series columns, a variety of solvents can be chosen ranging from aqueous solutions to organic solvents, making it possible to set the conditions for investigation based on the solubility of the chemical and the molecular weight standard. As a result, this series of columns can be used when analyzing polar polymer samples that have been so cumbersome to characterize with traditional SEC columns in the past.
SR028: Principles and Applications of High Performance GPC Columns: TSK-GEL Hxl Type Columns for Oligomer Analysis
In general, analysis by conventional GPC is more time consuming than separation by reversed phase or normal phase HPLC. The TSK-GEL Hxl type columns provide a significant improvement in this regard, by reducing analysis time over more conventional GPC column types. Using TSK-GEL Hxl type columns yields a number of major advantages, including reducing analysis time by half, increasing analysis performance, and decreased solvent consumption and disposal costs.
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Application Notes
AN17: TSK-GEL ODS-140HTP, 2.3µm: Ultra-High Performance Reversed Phase Columns for High Throughput Analysis
TSK-GEL ODS-140HTP, 2.3µm reversed phase HPLC columns provide high resolution and short analyses times at moderate pressures. For use in high throughput applications, including drug discovery, pharmacokinetics and peptide digest separations, these 2.3µm columns offer excellent resolution.
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