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Published Literature
Molecular and Cellular Proteomics: Hydrophilic-interaction chromatography reduces the complexity of the phosphoproteome and improves global phosphopeptide isolation and detection
Annan, R.S.; McNulty, D.E. Hydrophilic-Interaction Chromatography Reduces the Complexity of the Phosphoproteome and Improves Global Phosphopeptide Isolation and Detection. Mol. Cell. Proteomics. [Online early access].
DOI:10.1074/mcp.M700543-MCP200. Published online: Jan 22, 2008.
This paper reports on the use of Hydrophilic Interaction Chromatography (HILIC) as part of a multidimensional chromatography strategy for proteomics. Tryptic peptides are separated on TSK-GEL Amide-80 columns using a shallow inverse organic gradient.
Separation Reports
SR097: Application of TSK-GEL Semi-Micro Columns for High-Sensitivity Analyses
Semi-micro columns, also referred to as narrow bore columns, are the columns of choice when faced with a limited sample mass, as in trace analysis, or when using a mass spectrometer or other detector that performs more optimally at lower flow rates. Tosoh Bioscience offers an array of semi-micro columns that can all be used in a wide variety of applications due to their excellent performance and chemical stability. Ion exchange, reversed phase and hydrophobic interaction chromatography columns are discussed.
SR079: Separation of Saccharides Using TSK-GEL Amide-80, a Packing Material for High Performance Normal Phase Partition Chromatography, Part 2 |
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Normal phase chromatography separates saccharides based on differences in polarity. A packing material containing carbamoyl groups bonded to silica gel was developed and commercialized, TSK-GEL Amide-80, in order to overcome the poor chemical stability and low recovery of reducing sugars exhibited by amino-bonded silica gel packing material. This report compares TSK-GEL Amide-80 with other silica-type packing materials and examines the effect of organic solvents and amines in the mobile phase. Note: Part 1, SR055, can be found in the literature section of our website.
Application Notes
AN16: TSK-GEL PWxl-CP Size Exclusion Chromatography Columns for the Analysis of Water-Soluble Cationic Polymers
TSK-GEL PWxl-CP size exclusion columns eliminate ionic adsorption onto the particle by incorporating a cationic functionality on the particle surface. This modification results in high recovery for cationic polymers and enables elution under low salt conditions.
Product Overview
PO18: Toyopearl Phenyl-600M for high capacity Hydrophobic Interaction Chromatography and high recovery of large and small proteins
Toyopearl Phenyl-600M is a new hydrophobic interaction resin for process scale chromatography. It has high recovery and improved dynamic binding capacity for the intermediate and downstream purification of monoclonal antibodies.
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