TSKgel SuperSW mAb HR

The TSKgel SuperSW mAb HR column offers high resolution analysis of mAb fragments, monomer, and dimer. The high resolution capability of this column is due to a unique pore-controlled technology, which produces a shallow calibration curve in the molecular weight region of a typical monoclonal antibody.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0022854	TSKgel SuperSW mAb HR	4	Stainless Steel	7.8	30	2,321	Inquire

Product Attributes

Base material:	Silica
Particle size (mean):	4 µm
Pore size (mean):	25 nm
Functional group:	Diol
pH stability:	2.5-7.5
Calibration range:	1 × 10⁴ - 5 × 10⁵ Da (globular proteins)

Protein Calibration Curves

Comparison of Resolution of mAb Monomer and Dimer

Columns:	A. TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm B. Brand A, 3 µm, 7.8 mm ID × 30 cm C. Brand B, 5 µm, 7.8 mm ID × 30 cm D. Brand C, 5 µm, 8.0 mm ID × 30 cm E. Brand D, 5 µm 8.0 mm ID 30 cm
Mobile phase:	200 mmol/L phosphate buffer, pH 6.7 + 0.05% NaN₃
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	10 µL
Samples:	lgG (human polyclonal), 1.0 g/L

High Durability of TSKgel SuperSW mAb HR Column

Column:	TSKgel SuperSW mAb HR, 4 µm, 7.8 mm ID × 30 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN₃
Flow rate:	0.8 mL/min
Detection:	UV @ 280 nm
Injection vol.:	10 µL
Samples:	1. γ-Globulin 2. Cytochrome C 3. DNP-L-Alanine

Code #	Description	Literature Type
AN051	Separation of a Monoclonal Antibody Monomer from its Impurities using New TSKgel SW mAb Columns	Application Note
AN058	Separation of Monoclonal Antibody (mAb) Monomer from its Half-body using Size Exclusion Chromatography	Application Note
DS1239	TSKgel SW mAb Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01A	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series Columns	Product Catalog
PO35	TSKgel SuperSW mAb HR, TSKgel SuperSW mAb HTP and TSKgel UltraSW Aggregate Columns	Product Overview
TP166	New silica-based SEC columns designed for the separation of mAb monomers and their impurities	Technical Presentation
TP175	Three novel prototype SEC columns for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragments	Technical Presentation
TP180	Three novel prototype silica-based SEC columns designed for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragments	Technical Presentation
TP181	Detection of protein heterogeneity by HPLC (additional data included)	Technical Presentation
TP183	High Resolution Analysis of Monoclonal Antibody Heterogeneity by Size Exclusion Chromatography and Hydrophilic Interaction Chromatography	Technical Presentation
TP185	Three Novel Size Exclusion Chromatography Columns Designed for the Separation of a Monoclonal Antibody Monomer from its Impurities	Technical Presentation
TP195	Separation of Half-mAb and Half-mAb Equivalents with High Resolution using Size Exclusion Chromatography Packed with a Unique Controlled Pore Technology	Technical Presentation
TP197	Evaluation of Mobile Phase Conditions on the Resolution of Monoclonal Antibody Monomer from its Protein Variants Separated on a TSKgel SuperSW mAb HR SEC Column	Technical Presentation

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0022857	TSKgel SuperSW mAb HR Guard Column for 7.8 mm ID column	4	Stainless Steel	6	4	843