TSKgel SuperSW mAb HTP

The TSKgel SuperSW mAb HTP column is designed with the same pore-controlled technology as the TSKgel SuperSW mAb HR column and is specialized for fast run times. HTP stands for “high throughput,” and the TSKgel SuperSW mAb HTP column's reduced internal diameter and length (4.6 mm ID x 15 cm) allows for high resolution between the mAb dimer and monomer in less time. The resolution is equivalent to that of the TSKgel G3000SWxl column, but in half of the separation time.

The TSKgel SuperSW mAb HTP column is compatible with both HPLC and UHPLC systems because of its ability to withstand high flow rates and low pressure drops.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0022855	TSKgel SuperSW mAb HTP	4	Stainless Steel	4.6	15	1,730	Inquire

Product Attributes

Base material:	Silica
Particle size (mean):	4 µm
Pore size (mean):	25 nm
Functional group:	Diol
pH stability:	2.5-7.5
Calibration range:	1 × 10⁴- 5 × 10⁵Da (globular proteins)

Protein Calibration Curves

High Speed Separation of Therapeutic mAb

Columns:	TSKgel SuperSW mAb HTP 4 µm, 4.6 mm ID × 15 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.7 + 0.05% NaN₃
Flow rate:	0.50 mL/min, 0.35 mL/min
Detection:	UV @ 280 nm
Temperature:	25 °C
Injection vol.:	10 µL
Sample:	monoclonal antibody-2 (mouse-human chimeric IgG, Erbitux^®), 5 µL

Separation of Four Nucleobases Using TSKgel SuperSW mAb HTP Column in HILIC mode at pH 7.4

Column:	TSKgel SuperSW mAb HTP 4 µm, 4.6 mm ID × 15 cm
Mobile phase:	A: acetonitrile B: 15 mmol/L ammonium bicarbonate, pH 7.4
Gradient:	isocratic
Flow rate:	0.4 mL/min
Detection:	UV @ 280 nm
Injection vol.:	1 µL
Temperature:	ambient
Samples:	uracil (1.5 g/L), adenine (1.5 g/L), cytosine (1.5 g/L), cytodine (1.5 g/L), from Sigma Aldrich

For additional applications, please see our applications database.

Performance Stability of the TSKgel SuperSW mAb HTP Column Over 1000 Consecutive Injections of Protein Standard

Columns:	TSKgel SuperSW mAb HTP, 4 µm, 4.6 mm ID × 15 cm
Mobile phase:	100 mmol/L phosphate/100 mmol/L sodium sulfate, pH 6.7 + 0.05% NaN₃
Gradient:	isocratic
Flow rate:	0.35 mL/min
Detection:	UV @ 280 nm
Injection vol.:	5 µL (21.1 µg total protein load)
Samples:	protein standard: thyroglobulin, 0.58 g/L, γ-globulin, 1.02 g/L, ovalbumin, 1.08 g/L, ribonuclease, 1.53 g/L, PABA, 0.01 g/L

Code #	Description	Literature Type
AN047	Improved SEC Separations using TSKgel Columns on a UHPLC System	Application Note
AN064	Separation of Nucleobases using TSKgel SuperSW mAb HTP column in HILIC mode	Application Note
AN074	Evaluating the Column Lifetime of TSKgel SuperSW mAb HTP	Application Note
AN077	High Throughput Sub-4 Minute Separation of Antibodies using Size Exclusion Chromatography	Application Note
DS1239	TSKgel SW mAb Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01A	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series Columns	Product Catalog
PO35	TSKgel SuperSW mAb HR, TSKgel SuperSW mAb HTP and TSKgel UltraSW Aggregate Columns	Product Overview
TP166	New silica-based SEC columns designed for the separation of mAb monomers and their impurities	Technical Presentation
TP175	Three novel prototype SEC columns for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragments	Technical Presentation
TP180	Three novel prototype silica-based SEC columns designed for the separation of an antibody monomer from its dimer, higher aggregates, and antibody fragments	Technical Presentation
TP181	Detection of protein heterogeneity by HPLC (additional data included)	Technical Presentation
TP185	Three Novel Size Exclusion Chromatography Columns Designed for the Separation of a Monoclonal Antibody Monomer from its Impurities	Technical Presentation
TP205	High Throughput and Highly Reproducible Sub-4 Minute Separation of Proteins and Antibodies using Size Exclusion Chromatography	Technical Presentation
TP212	Overview of the HILIC Mode of Chromatography for the Analysis of Polar Analytes	Technical Presentation

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0022858	TSKgel SuperSW mAb HTP Guard Column for 7.8 mm ID column	4	Stainless Steel	3	2	644