TSKgel G2000SW

As the first generation of silica-based SEC columns, TSKgel G2000SW columns provide excellent separation of peptides and proteins with molecular weights up to 100,000 Da.

TSKgel G2000SW columns are available in 10 and 13 μm for analytical and semi-preparative purifications.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0005788	TSKgel G2000SW	10	Stainless Steel	7.5	30	1,987	Inquire
0005102	TSKgel G2000SW	10	Stainless Steel	7.5	60	3,251	Inquire
0006727	TSKgel G2000SW	13	Stainless Steel	21.5	30	5,521	Inquire
0005146	TSKgel G2000SW	13	Stainless Steel	21.5	60	8,023	Inquire

Product Attributes

Base material:	Silica
Particle size (mean):	10 µm, 13 µm
Pore size (mean):	12.5 nm
Functional group:	Diol
pH stability:	2.5-7.5
Calibration range:	5,000 - 1.0 × 10⁵Da (globular proteins)

Calibration Curves for Globular Proteins, Polyethylene Oxides and Dextrans
for TSKgel SW Columns

Columns:	TSKgel SW columns, 7.5 mm ID × 60 cm × 2
Mobile phase:	dextrans and polyethylene oxides: distilled water; proteins: 0.3 mol/L NaCl in 0.1 mol/L phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV @ 220 nm and RI
Temperature:	A. 25°C B. 25°C C. 40°C
Samples:	proteins (black circle), polyethylene oxides (white circle), dextrans (half circle)

Separation of Nucleic Acid

Column:	TSKgel G2000SW, 10 µm, 7.5 mm ID × 60 cm
Mobile phase:	acetic acid/triethylamine/H₂O = 3/3/94
Flow rate:	0.74 mL/min
Detection:	UV @ 260 nm
Sample:	1. uridine 2. uracil 3. thymine 4. adenosine 5. adenine

Comparison of the Pore Sizes of TSKgel SW Packings for the Separation of a Protein Mixture

Columns:	TSKgel SW columns, two 7.5 mm ID × 60 cm columns in series
Mobile phase:	0.3 mol/L NaCl in 0.05 mol/L phosphate buffer, pH 7.0
Flow rate:	1.0 mL/min
Detection:	UV @ 220 nm
Samples:	1. thyroglobulin, 2. bovine serum albumin, 3. β-lactoglobulin, 4. myoglobin, 5. cytochrome C, 6. glycine tetramer

For additional applications, please see our applications database.

Code #	Description	Literature Type
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
AO02	Purification of Nucleic Acids	Application Overview
DS1005	TSKgel G2000SW Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01A	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series Columns	Product Catalog
SR024	Applications for High-Performance Gel Filtration Columns (TSKgel SW and TSKgel PW columns): Focus on Biopolymers	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0005371	TSKgel G2000SW-G4000SW Guard Column for 7.5 mm ID columns	10	Stainless Steel	7.5	7.5	748
0005758	TSKgel G2000SW-G4000SW Guard Column for 21.5 mm ID columns	13	Stainless Steel	21.5	7.5	1676