TSKgel G5000PWxl

The TSKgel G5000PWxl column is ideally suited for analysis of water-soluble linear polymers with molecular weights up to 1 × 10⁶ Da. The smaller particle size of the TSKgel G5000PWxl column provides 1.7× higher resolution than the TSKgel G5000PW column, making the TSKgel G5000PWxl column more suitable for analytical purposes.

The TSKgel G5000PWxl column has a base material of hydroxylated polymethacrylate, can be used in a maximum of 20% organic, and is shipped in water.

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008023	TSKgel G5000PWxl	10	Stainless Steel	7.8	30	2,341	Inquire

Product Attributes

Base material:	hydroxylated polymethacrylate
Particle size (mean):	10 µm
Pore size (mean):	100 nm
Calibration range:	4,000 - 8.0 × 10⁵ Da (polyethylene glycols and oxides)

PEG and PEO Calibration Curves

Columns:	A. G2500PWXL B. G3000PWXL C. G4000PWXL D.G5000PWXL E. G6000PWXL F. GMPWXL all 7.8 mm ID × 30 cm
Mobile phase:	distilled H₂O
Flow rate:	1.0 mL/min
Detection:	Rl

Protein Calibration Curves

Columns:	1. TSKgel G3000PWXL 2. TSKgel G4000PWXL 3. TSKgel G5000PWXL 4. TSKgel G6000PWXL 5. TSKgel GMPWXL all 7.8 mm ID × 30 cm
Mobile phase:	0.2 mol/L phosphate buffer, pH 6.8
Flow rate:	1.0 mL/min
Detection:	UV @ 280 nm
Samples:	a. thyroglobulin (6.6 × 10⁵Da) b. γ-globulin (1.5 × 10⁵Da) c. albumin (6.7 × 10⁴Da) d. ovalbumin (4.3 × 10⁴Da) e. β-lactoglobulin (3.6 × 10⁴Da) f. myoglobin (1.69 × 10⁴Da) g. cytochrome C (1.24 × 10⁴Da)

Code #	Description	Literature Type
AN125	Molecular Weight Determination of VLPs Using LenS3 Multi-Angle Light Scattering Detector	Application Note
AO01	Gel Filtration Chromatography with TSKgel columns	Application Overview
AO02	Purification of Nucleic Acids	Application Overview
DS1025	TSKgel G5000PWxl Products	Operating Conditions and Specifications
IM01	TSKgel Column Instruction Manual	Instruction Manual
LCAT01B	TSKgel U/HPLC Columns 2019 Product Guide -- SEC, PW Series Columns	Product Catalog
SR037	Introduction of Aqueous SEC Columns: TSKgel PWxl series	Separation Report
SR038	GFC Separation of Water-Soluble Polymers using TSKgel PWxl Series Columns	Separation Report

P/N	Description	Particle Size (µm)	Housing Material	ID (mm)	Length (cm)	Price ($)
0008033	TSKgel G2500PWxl-GMPWxl, and G-DNA-PW Guard Column for 7.8 mm ID columns	12	Stainless Steel	6	4	798

HPLC Columns

TSKgel G5000PWxl

Product Attributes

PEG and PEO Calibration Curves

Protein Calibration Curves