Analysis of Standard Proteins at Low Level Concentrations
| Columns: |
TSKgel SuperSW3000, 4 µm,
2 mm ID × 30 cm
Competitor 200 PC 3.2/30, 13 µm,
3.2 mm ID × 30 cm |
| Mobile phase: |
0.1 mol/L phosphate buffer + 0.1 mol/L Na2SO4 + 0.05% NaN3, pH 6.7 |
| Detection: |
UV @ 280 nm |
| Injection vol.: |
0.2 µL
|
| Samples: |
1. thyroglobulin (1.0 g/L)
2. β-globulin (2.0 g/L)
3. ovalbumin (2.0 g/L)
4. ribonuclease A (3.0 g/L)
5. p-aminobenzoic acid (0.02 g/L) |
|
|
Analysis of Proteins in Human Serum
| Column: |
TSKgel SuperSW3000, 4 µm,
1 mm ID × 30 cm |
| Mobile phase: |
50 mmol/L NaH2PO4 + 0.5 mol/L NaCl, pH 7.0 |
| Flow rate: |
8 µL/min |
| Detection: |
UV @ 280 nm |
| Temperature: |
ambient |
| Sample: |
serum (x 10), 1 µL |
Fraction (1 mL) was directly loaded to SELDI chip H50. The chip was washed and desalted then applied to MS.
This data is courtesy of Dr. Majima, Protenova
|
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