TOYOPEARL SuperQ-650M

TOYOPEARL SuperQ-650M resin is a strong anion exchanger for biomolecule purification. This resin is composed of a base material of hydroxylated methacrylic polymer beads that have been functionalized with quaternary amine (Q) strong anion exchange groups. This resin has a 100 nm pore size and a 65 µm bead size.

TOYOPEARL SuperQ-650M resin is best used for high throughput intermediate purification and polishing process steps. If greater resolution is needed, the smaller particle size TOYOPEARL SuperQ-650S or TSKgel SuperQ-5PW resins may be used. As both the TOYOPEARL SuperQ-650 and TSKgel SuperQ-5PW resins use the same ligand and backbone chemistry, the selectivity will be unchanged between the two resin types.

P/N	Description	Resin Volume	Particle Size (µm)	Price ($)
0043205	TOYOPEARL SuperQ-650M	100 mL	65	323	Inquire
0017227	TOYOPEARL SuperQ-650M	250 mL	65	734	Inquire
0017228	TOYOPEARL SuperQ-650M	1 L	65	-	Inquire
0017229	TOYOPEARL SuperQ-650M	5 L	65	-	Inquire
0021311	TOYOPEARL SuperQ-650M	50 L	65	-	Inquire

Product Attributes

Base bead:	HW-65
Pore size (mean):	100 nm
Particle size (mean):	65 µm
Ligand type:	strong anion
Ligand pKa:	12.2
DBC:	105 - 155 g/L
Shipping buffer:	20% ethanol
pH stability:	2-13
Stability data:	10 years

Structure:

Dynamic Binding Capacity Comparision of AIEX Resins for BSA @ 100 cm/hr

Resins:	binding capacity (10% breakthrough)
TOYOPEARL DEAE-650M	40.1 g/L
TOYOPEARL QAE-550C	81.3 g/L
TOYOPEARL SuperQ-650M	128.8 g/L

Benzyl Alcohol Removal

A 2% solution of benzyl alcohol in water has been identified as a suitable alternative to 20% ethanol as a preservative in resin storage solutions. A sample of TOYOPEARL SuperQ-650M resin was prepared by adding 100 mL of aqueous 2% benzyl alcohol to 100 mL of suction filtered resin. A 100 mL aliquot of DI water was added to the filtered resin and stirred to make a slurry. This resin/ DI water slurry was allowed to stand for 5 minutes and was then suction filtered to remove the supernatant. This procedure was repeated 14 more times, for a total of 15 washes.

Samples of the filtered supernatant from the TOYOPEARL SuperQ-650M resin was taken after the 5th, 10th, and 15th washes and analyzed for benzyl alcohol concentration. As demonstrated in the figure, a 2% benzyl alcohol solution can be removed from the TOYOPEARL SuperQ-650M resin by thorough washing with DI water.

Scale Up or Down using the Same Ligand

Resins:	A. TOYOPEARL SuperQ-650C, 100 µm B. TOYOPEARL SuperQ-650M, 65 µm C. TOYOPEARL SuperQ-650S, 35 µm D. TSKgel SuperQ-5PW (30), 30 µm E. TSKgel SuperQ-5PW (20), 20 µm F. TSKgel SuperQ-5PW, 10 µm
Column size:	7.5 mm ID × 7.5 cm
Mobile phase:	Buffer A: 0.02 mol/L Tris-HCl, pH 8.5 Buffer B: 0.5 mol/L NaCl in buffer A
Gradient:	60 min linear gradient from buffer A to buffer B
Flow rate:	136 cm/hr (1.0 mL/min)
Detection:	UV @ 280 nm
Sample:	mAb in mouse ascites (dilution, × 5)
Load volume:	100 µL

Code #	Description	Literature Type
AN024	Preserving Chromatographic Selectivity While Improving Your Process Purification	Application Note
AN056	Clearance of 2% Benzyl Alcohol from TOYOPEARL Resins	Application Note
IM02	Instruction Manual for TOYOPEARL and TSKgel PW-type Resins	Instruction Manual
PCAT02	TOYOPEARL, TSKgel and Ca⁺⁺Pure-HA Bulk Resin 2019 Product Guide -- Ion Exchange	Product Catalog
PD16	Stability of TOYOPEARL Ion Exchange and Hydrophobic Interaction Chromatography Resins Stored in 2% Benzyl Alcohol	Performance Data
RIS0029	TOYOPEARL SuperQ-650	Resin Information Sheet
TP138	One-step oligonucleotide purifications using anion-exchange chromatography resins	Technical Presentation

P/N	Description	Price ($)
0045109	MiniChrom TOYOPEARL SuperQ-650M	-	Inquire
0043210	TOYOPEARL AIEXPAK	461	Inquire
0021392	ToyoScreen IEC Anion Mix Pack, 1 mL (qty. 5)	-	Inquire
0021393	ToyoScreen IEC Anion Mix Pack, 5 mL (qty. 5)	-	Inquire
0021396	ToyoScreen IEC Mix Pack, 1 mL (qty. 5)	-	Inquire
0021397	ToyoScreen IEC Mix Pack, 5 mL (qty. 5)	-	Inquire
0021362	ToyoScreen SuperQ-650M, 1 mL (qty. 6)	-	Inquire
0021363	ToyoScreen SuperQ-650M, 5 mL (qty. 6)	-	Inquire