Products

HPLC Columns

TSKgel BioAssist Q

TSKgel BioAssist Q columns are packed with polymethacrylate particles containing very large pores (~400 nm) that are functionalized with polyamine groups to form a network structure. The very large pores allow large biomolecules to freely enter and exit the pores resulting in higher binding capacity and better recovery of activity than other competitive materials. The capacity of the TSKgel BioAssist Q columns is high over a wide molecular weight range (up to 1.0 × 106 Da).

The standard TSKgel BioAssist Q column is 4.6 mm ID × 5 cm, while a 10 mm ID × 10 cm semi-preparative column is available for scale-up. Both columns are made of PEEK to reduce protein adsorption.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
19685TSKgel BioAssist Q10PEEK4.651,258
21410TSKgel BioAssist Q13PEEK10104,802
        

Product Attributes

Matrix: polymethacrylate
Particle size (mean): 10 µm and 13 µm
Pore size (mean): 400 nm
Functional group: polyamine
Counter ion: Cl-
pH stability: 2.0 - 12.0
Capacity (mg BSA/mL): 70
Small ion capacity: 0.1 eq/L
pKa: 9.4

 

 

 

 


        

Analysis of Mouse Ascites Fluid

 

IEX_BioAssist-Q-Fig2.png

Columns: A: TSKgel BioAssist Q, 10 µm,
4.6 mm ID × 5 cm

B: Commercial Q-type product A, 5.0 mm ID × 5 cm
Mobile phase: 15 min linear gradient of NaCl from 0 to 1.0 mol/L in 20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Injection vol.: 5 µL
Sample: mouse ascites fluid (3-fold dilution with initial eluent)

 

For additional applications, please see our applications database.

 
 
                

Performance Enhancement on FPLC System

IEX_BioAssist-Q-Fig3.png

 

Columns: A: TSKgel BioAssist Q, 10 µm, 4.6 mm ID × 5 cm
B: Competitor Q, 5.0 mm ID × 5 cm 
Mobile phase: 30 min linear gradient from 0 to 1 mol/L NaCl in 20 mmol/L sodium phosphate, pH 8.0
Flow rate: 1.0 mL/min
Detection: UV @ 280 nm
Samples: 1. conalbumin
i. ovalbumin impurity
2. ovalbumin
3. trypsin inhibitor

 

Dynamic Binding Capacity as function of Protein Molar Mass

IEX_BioAssist-Q-Fig4.png

 

Column: TSKgel BioAssist Q, 10 µm,
4.6 mm ID × 1 cm
Conventional Q-type product A, 5.0 mm ID × 1 cm
Mobile phase: 20 mmol/L Tris-HCl buffer, pH 8.0
Flow rate: 0.38 mL/min
Detection: UV @ 280 nm
Temperature: 25 °C
Samples: 1. trypsin inhibitor, 10 g/L
2. human serum albumin, 10 g/L
3. IgG1, 2.3 g/L
4. thyroglobulin, 5 g/L

For additional applications, please see our applications database.

 

Code #DescriptionLiterature Type
AN008 Ion Exchange Analysis of Monoclonal Antibodies on Large Pore TSKgel BioAssist ColumnsApplication Note
DS1183 TSKgel BioAssist Q ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT02A TSKgel HPLC/UHPLC Columns 2017 Product Guide -- AIEC ColumnsProduct Catalog
PO04 TSKgel BioAssist Ion Exchange ColumnsProduct Overview
SR100 TSKgel BioAssist Series Ion Exchange ColumnsSeparation Report
TP100 Purification of Immunoglobulin M using a novel ion-exchanger (BioAssist Q and S)Technical Presentation
TP102 The Characteristics of Novel Semi-Preparative Ion-Exchange ColumnsTechnical Presentation