Solutions

Anion Exchange

TSKgel Anion Exchange Chromatography Columns

Very efficient chromatography can be achieved with the nonporous TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel Q-STAT columns include the separation of: proteins, peptides, low molecular weight nucleic acids, aggregates and charge isomers of monoclonal antibodies, PEGylated proteins, oligo DNA, and siRNA. The TSKgel DNA-STAT column is ideal for the analysis of DNA fragments, nucleic acids and nucleotides.

The pore structure and bonding chemistry of TSKgel BioAssist Q columns provides high capacity for small to very large molar mass proteins and nucleic acids.

Proteins, peptides, DNA and RNA-derived oligonucleotides, and other nucleic acid fragments are typical samples that are analyzed or isolated on the TSKgel DEAE-5PW and TSKgel SuperQ-5PW columns.

TSKgel DEAE-NPR nonporous columns are used for the high speed separation and analysis of proteins and poly and oligonucleotides. Applications for the TSKgel DNA-NPR nonporous columns include the high efficiency separation of DNA fragments, PCR products, or plasmids.

TSKgel DEAE-2SW and TSKgel QAE-2SW (both 5 µm, 12.5 nm) and TSKgel DEAE-3SW (10 µm, 25 nm) are available for analyzing smaller molar mass samples such as nucleotides, drug candidates, catecholamines, and small peptides or proteins.

TSKgel Sugar AXG columns are packed with 10 µm beads and are used for the gradient separation and analysis of monosaccharides, disaccharides, and sugar alcohols.

For isocratic separation of carbohydrates where lower and constant back pressures may be generated, the 8 µm TSKgel Sugar AXI column is optimal.

TSKgel SAX columns are used for the separation of isomerized sugars, alcohols, and low molar mass organic acids.



Base Material:
polymethacylate
Particle Size (mean): 10 µm and 13 µm
 
Pore Size (mean): 400 nm  
Functional Group: polyamine                   Visit the TSKgel BioAssist Q product page.
Counter Ion: Cl-          
pH Range: 2.0 - 12.0
Capacity
(mg BSA/mL):
70  
Small Ion Capacity: 0.1 meq/mL  
pKa: 9.4  


Base Material: hydrophilic silica
Particle Size (mean): µm
 
Pore Size (mean): 12.5 nm  
Functional Group: CH2CH2N+(C2H5)3
                  Visit the TSKgel DEAE-2SW product page.
Counter Ion: H2PO4-
         
pH Range: 2.0 - 7.5
Capacity 
(mg BSA/mL):
ND  
Small Ion Capacity: >0.3 meq/mL
pKa: 11.2


Base Material: hydrophilic silica
Particle Size (mean): 10 µm
 
Pore Size (mean): 25 nm  
Functional Group: CH2CH2N+(C2H5)3
                  Visit the TSKgel DEAE-3SW product page.
Counter Ion: Cl-          
pH Range: 2.0 - 7.5
Capacity 
(mg BSA/mL):
ND  
Small Ion Capacity: >0.3 meq/mL  
pKa: 11.2  



Base Material: hydroxylated methacrylate 
polymer

Particle Size (mean): 10, 13 and 20 µm
 
Pore Size (mean): 100 nm  
Functional Group: CH2CH2N+(C2H5)3
                 Visit the TSKgel DEAE-5PW product page.
Counter Ion: Cl-
         
pH Range: 2.0 - 12.0
Capacity 
(mg BSA/mL):
30  
Small Ion Capacity: 0.1 meq/mL  
pKa: 11.5  



Base Material: hydroxylated methacrylate 
polymer

Particle Size (mean): 2.5 µm
 
Pore Size (mean): non-porous  
Functional Group: CH2CH2N+(C2H5)3
                Visit the TSKgel DEAE-NPR product page.
Counter Ion: Cl-
         
pH Range: 2.0 - 12.0
Capacity 
(mg BSA/mL):
5  
Small Ion Capacity: >0.1 meq/mL  
pKa: 11.2  



Base Material: hydroxylated methacrylate 
polymer


Particle Size (mean): 2.5 µm
 
Pore Size (mean): non-porous  
Functional Group: proprietary
                Visit the TSKgel DNA-NPR product page.
Counter Ion: ClO4-
         
pH Range: 2.0 - 12.0
Capacity 
(mg BSA/mL):
5  
Small Ion Capacity: 0.1 meq/mL  
pKa: 11.2  



Base Material: hydrophilic polymer

Particle Size (mean): µm
 
Pore Size (mean): non-porous  
Functional Group: quarternary ammonium
                 Visit the TSKgel DNA-STAT product page.
Counter Ion: Cl          
pH Range: 3.0 - 10.0
Static Binding Capacity 
(mg BSA/g dry gel):
ca. 35 (5 µm)
 
Small Ion Capacity: 270 µeg/g dry gel
 
pKa: 10.5  

Hardware: Stainless Steel
tubing & fittings,
PEEK frits



Base Material: silica

Particle Size (mean): µm
 
Pore Size (mean): 12.5 nm  
Functional Group: trimethylamino
                  Visit the TSKgel QAE-2SW product page.
Counter Ion: H2PO4-
         
pH Range: 2.0 - 7.5
Capacity 
(mg BSA/mL):
ND  
Small Ion Capacity: >0.3 meq/mL  
pKa: 11.2  



Base Material: hydrophilic polymer

Particle Size (mean): µm and 10 µm
 
Pore Size (mean): non-porous  
Functional Group: quarternary ammonium
                  Visit the TSKgel Q-STAT product page.
Counter Ion: Cl          
pH Range: 3.0 - 10.0
Static Binding Capacity 
(mg BSA/g dry gel):
ca. 35 (7 µm)
ca. 20 (10 µm)
 
Small Ion Capacity: 270 µeg/g dry gel
 
pKa: 10.5  

Hardware: Stainless Steel
tubing & fittings, 
PEEK frits
 



Base Material: PS-DVB polymer

Particle Size (mean): µm
 
Pore Size (mean): 6.0 nm  
Functional Group: trimethylamine
                  Visit the TSKgel SAX product page.
Counter Ion: Cl-          
pH Range: 1.0 - 14.0
Capacity 
(mg BSA/mL):
ND  
Small Ion Capacity: >0.1 meq/mL  
pKa: 12.5  



Base Material: PS-DVB polymer

Particle Size (mean): 10 µm
 
Pore Size (mean): 6.0 nm  
Functional Group: trimethylamino
                  Visit the TSKgel Sugar AXG product page.
Counter Ion: HBO3-          
pH Range: 1.0 - 14.0
Capacity 
(mg BSA/mL):
NA  
Small Ion Capacity: >1.2 meq/mL  
pKa: 12.5



Base Material: PS-DVB polymer

Particle Size (mean): µm
 
Pore Size (mean): 6.0 nm  
Functional Group: trimethylamino
                  Visit the TSKgel Sugar AXI product page.
Counter Ion: HBO3-          
pH Range: 1.0 - 14.0
Capacity 
(mg BSA/mL):
ND  
Small Ion Capacity: >1.2 meq/mL  
pKa: 12.5  



Base Material: polymethacrylate

Particle Size (mean): 10 µm and 13 µm
 
Pore Size (mean): 100 nm  
Functional Group: trimethylamino
                  Visit the TSKgel SuperQ-5PW product page.
Counter Ion: Cl-          
pH Range: 2.0 - 12.0
Capacity 
(mg BSA/mL):
100  
Small Ion Capacity: >0.13 meq/mL  
pKa: 12.2