Solutions

Cation Exchange Chromatography

TSKgel Cation Exchange Chromatography Columns

Very efficient chromatography can be achieved with the nonporous TSKgel STAT columns due to the novel bonding chemistry and the absence of micro-pores. Applications for the TSKgel CM-STAT and TSKgel SP-STAT columns include the separation of proteins, protein aggregates,charge variants of monoclonal antibodies, PEGylated proteins, and peptide digests.

The pore structure and bonding chemistry of TSKgel BioAssist S columns provides high capacity for medium to large molar mass proteins.

TSKgel CM-5PW and TSKgel SP-5PW columns are an excellent choice for analyzing biologically active molecules.

TSKgel SP-NPR
nonporous columns are used for the separation and analysis of proteins and peptides. They are particularly useful for adeno-associated viruses and other large macromolecules.

TSKgel CM-2SW and TSKgel SP-2SW (both 5 µm, 12.5 nm) and TSKgel CM-3SW (10 µm, 25 nm) are available for analyzing smaller molar mass samples such as nucleotides, drug candidates, catecholamines, and small peptides or proteins.

TSKgel SCX columns are used for the separation and analysis of organic acids, saccharides and alcohols.

TSKgel OApak-A
columns are optimized for the separation and analysis of organic acids by an ion-exclusion mechanism. Applications include: organic acids in fruit juices, wine, beer, coffee, and salt solutions.


Base Material: polymethacrylate
Particle Size (mean): µm and 13 µm
 
Pore Size (mean): 130 nm  
Functional Group: sulfopropyl                   Visit the TSKgel BioAssist S product page.
Counter Ion: Na+          
pH Range: 2.0 - 12.0
Capacity 
(gamme globulin):
70  
Small Ion Capacity: 0.1 meq/mL
pKa: 2.4


Base Material: spherical silica
Particle Size (mean): 5 µm
 
Pore Size (mean): 12.5 nm  
Functional Group: -CH2COO-                             Visit the TSKgel CM-2SW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):
110  
Small Ion Capacity: >0.3 meq/mL
pKa: 4.2


Base Material: spherical silica
Particle Size (mean): 10 µm
 
Pore Size (mean): 25 nm  
Functional Group: -CH2COO-
                            Visit the TSKgel CM-3SW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):
ND  
Small Ion Capacity: >0.3 meq/mL
pKa: 4.2


Base Material: hydroxylated methacrylic
polymer

Particle Size (mean): 10 µm and 13 µm
 
Pore Size (mean): 100 nm  
Functional Group: -CH2COO-
                            Visit the TSKgel CM-5PW product page.
Counter Ion: Na+          
pH Range: 2.0 - 12.0
Capacity 
(mg Hb/mL):
45  
Small Ion Capacity: >0.1 meq/mL
pKa: 4.2


Base Material: hydrophilic polymer
Particle Size (mean): 7 µm and 10 µm
 
Pore Size (mean): non-porous  
Functional Group: carboxymethyl                Visit the TSKgel CM-STAT product page.
Counter Ion: Na          
pH Range: 3.0 - 10.0
Static Binding Capacity 
(mg Lysozyme/g dry gel):
ca. 20 (7 µm)
ca. 15 (10 µm)
 
Small Ion Capacity: 100 µeq/g dry gel
pKa: 4.9
Hardware: stainless steel
tubing and fittings, PEEK frits
 


Base Material: hydrophilic polymer
Particle Size (mean): 5 µm
 
Pore Size (mean): ND  
Functional Group: proprietary                             Visit the TSKgel OApak-A product page.
Counter Ion: H+          
pH Range: 2.0 - 7.5
Small Ion Capacity: >1.5 meq/mL


Base Material: PS-DVB
Particle Size (mean): 5 µm
 
Pore Size (mean): 6.0 nm  
Functional Group: sulfonic acid                   Visit the TSKgel SCX (H+) or TSKgel SCX (Na+) product page.
Counter Ion: H+ and Na+          
pH Range: 2 - 12
Small Ion Capacity: >1.5 meq/mL


Base Material: spherical silica
Particle Size (mean): 5 µm
 
Pore Size (mean): 12.5 nm  
Functional Group: sulfopropyl                             Visit the TSKgel SP-2SW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):
ND  
Small Ion Capacity: >0.3 meq/mL
pKa: 2.2


Base Material: hydroxylated methacrylic
polymer

Particle Size (mean): 10, 13, 20 µm
 
Pore Size (mean): 100 nm  
Functional Group: -(CH2)SO3-
                   Visit the TSKgel SP-5PW product page.
Counter Ion: Na+          
pH Range: 2.0 - 7.5
Capacity 
(mg Hb/mL):
40  
Small Ion Capacity: >0.1 meq/mL
pKa: 2.3

Base Material: hydroxylated methacrylic
 polymer

Particle Size (mean): 2.5 µm
 
Pore Size (mean): non-porous  
Functional Group: -(CH2)SO3-
                         Visit the TSKgel SP-NPR product page.
Counter Ion: Na+
         
pH Range: 2.0 - 12.0
Capacity 
(mg Hb/mL):
5  
Small Ion Capacity: >0.1 meq/mL  
pKa: 2.3


Base Material: hydrophilic polymer

Particle Size (mean): µm and 10 µm
 
Pore Size (mean): non-porous  
Functional Group: sulfopropyl                    Visit the TSKgel SP-STAT product page.
Counter Ion: Na          
pH Range: 3.0 - 10.0
Static Binding
Capacity (mg
Lysozyme/g dry gel):
ca. 15 (7 µm)
ca. 10 (10 µm)
 
Small Ion Capacity: 23 µeg/g dry gel
 
pKa: 4.0  

Hardware: Stainless Steel
tubing & fittings, 
PEEK frits