Solutions

Capture

Capture Process Step

A chromatographic "capture" or "concentration" step is a column just downstream of the harvested feedstock after initial clarification. Its primary function is to "capture" the target from solution and separate it from the most dominant impurities. The target is then eluted into a significantly smaller volume of buffer for further downstream processing. A resin is selected which has the best combination of dynamic binding capacity, mass recovery, and retention of the target's biological activity.

Column dimensions and resin particle sizes tend to be larger in capture steps. The feedstock viscosity, the amount of particulate, and the operational pressure limits of the chromatographic hardware used, determine the most appropriate particle size for a capture step. The more viscous and particulate laden the feedstock, the larger the particle needed to produce a linear velocity suitable for the process throughput objectives.

The typical capture step for monoclonal antibodies (mAbs) is based on Protein A affinity chromatography. This affinity step provides a high selectivity and yields a high purity of the target molecule in one step. By applying latest resin technology the dynamic binding capacity could be raised to more than 70 g IgG or mAb per liter Protein A resin. As raw treated feedstocks have become more clarified and less viscous, there is a trend to use smaller particle materials for capturing.

Many commercial TOYOPEARL and TSKgel PW bulk resin products are offered in particle sizes from 100 µm to 20 µm and have the same methacrylic polymer backbone. The selectivity of feedstock components on these resins remains the same regardless of bead size.

 

Tosoh Bioscience has commercial products with the following particle sizes for a typical capture step:

Grade

Particle Size Range

EC (Extra Course) 100 - 300 µm
C (Course) 50 - 150 µm
M (Medium) 40 - 90 µm
F (Fine) 30 - 60 µm

 

Approximate dynamic binding capacities for capture resins:

Mode

Typical Protein

Antibody

Ion Exchange up to 200 mg/mL
 up to 150 mg/mL
Hydrophobic Interaction up to 60 mg/mL
 up to 45 mg/mL
Protein A
n/a
 up to 65 mg/mL

Please click on the mode of interest for additional product information.

Chromatography Mode

Particle Size

TOYOPEARL Resin

Anion Exchange
100 µm
SuperQ-650C
DEAE-650C
QAE-550C
Q-600C AR
Anion Exchange
75 µm
GigaCap Q-650M
GigaCap DEAE-650M
Cation Exchange
200 µm
MegaCap II SP-550EC
Cation Exchange 100 µm
SP-650C
SP-550C
CM-650C
Cation Exchange 75 µm
GigaCap S-650M
GigaCap CM-650M
Hydrophobic Interaction
100 µm SuperButyl-550C
Hexyl-650C
Butyl-650C
Phenyl-650C
Protein A
45 µm
AF-rProtein A HC-650F
AF-rProtein A-650F