Products

HPLC Columns

TSKgel BioAssist G3SWxl

TSKgel BioAssist G3SWxl is the PEEK format of the TSKgel G3000SWxl column. These columns are an excellent choice for protein and large peptide separations.  The fractionation range is applicable for globular protein samples up to 0.5 million Da. These columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
20026TSKgel BioAssist G3SWxl5PEEK7.8301,775
           

Product Attributes

Base material: Silica
Particle size (mean): 5 µm
Pore size (mean): 25 nm
Functional group: Diol
pH stability: 2.5-7.5
Calibration range: 1.0 × 104- 5.0 × 10Da (globular proteins)

Calibration Curves

SEC_G2000SWxl-Calibration-rev.png

Column: TSKgel SWXL  columns,
7.8 mm ID × 30 cm
Mobile phase: 0.3 mol/L NaCl in 0.1 mol/L sodium phosphate buffer, pH 7.0
Detection: UV @ 220 nm
Samples: 1. thyroglobulin (6.6 × 10Da)
2. IgG (1.56 × 10Da)
3. bovine serum albumin
(6.7 × 10Da)
4. ovalbumin (4.3 × 10Da)
5. peroxidase(4.02 × 10Da)
6. β-lactoglobulin (3.5 × 10Da)
7. myoglobin (1.69 × 10Da)
8. ribonuclease A (1.37 × 10Da)
9. cytochrome C (1.24 × 10Da)
10. glycine tetramer (246 Da)

       

Purity of an Antibody

 

SEC_G3000SWxl-Fig16.png

 

Columns: TSKgel BioAssist G3SWXL, 5 µm, 
7.8 mm ID × 30 cm
Mobile phase: 0.3 mol/L phosphate buffer, pH 7.0
Flow rate: 1.0 mL/min
Injection vol.: 50 µL

For additional applications, please see our applications database.

 
Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01A TSKgel U/HPLC Columns 2019 Product Guide -- SEC, SW Series ColumnsProduct Catalog
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
18008TSKgel BioAssist G2SWxl-G4SWxl Guard Column for 7.8 mm ID columns7PEEK64627