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HPLC Columns

TSKgel Butyl-NPR

TSKgel Butyl-NPR is based on a non-porous polymethacrylate base material with uniform 2.5 µm particle size. It is the least hydrophobic among the TSKgel HIC columns and requires a higher salt concentration for binding.

TSKgel Butyl-NPR is the best choice for high speed separations with excellent recovery even for more hydrophobic samples. The non-porous resin requires lower sample loading and leads to faster analysis time. TSKgel Butyl-NPR is particularly popular for the analysis of drug to antibody ratios (DARs) of antibody drug conjugates (ADCs).

NPR stands for Non-Porous Resin.

 

 
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
14947TSKgel Butyl-NPR2.5Stainless Steel4.63.51,205
42168TSKgel Butyl-NPR2.5Stainless Steel4.6101,501

          

Product Attributes

Pore size (mean): non-porous
Particle size (mean): 2.5 µm
pH stability: 2.0-12.0
Functional group: butyl

Structure:

HIC_ButylNPR_structure.png

           

Determination of Drug to Antibody Ratio
Using a TSKgel Butyl-NPR Column

 

HIC_Butyl-NPR-FigX_pg114.png

 

Republished with permission of Genentech, Inc., from Analytical methods for physicochemical characterization of antibody drug conjugates, Wakankar A, Chen Y, Gokarn Y, Jacobson FS., 3, 2011; permission conveyed through Copyright Clearance Center, Inc.


Analysis of Monclonal Antibody and Aggregates Using a TSKgel Butyl-NPR column


HIC_Butyl-NPR-Fig2B.png

 

Column: TSKgel Butyl-NPR, 2.5 μm,
4.6 mm ID × 3.5 cm

Mobile phase: A: 3 mol/L NaCl
B: H2O
Gradient: 0-100% B in 10 min
Flow rate: 1.0 mL/min
Detection: flourescence; Ex: 280 nm, Em: 348 nm
Injection vol.: 5 μg
Sample: lgG1

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AN092 DAR Analysis of Antibody Drug Conjugates using a TSKgel HIC Column Application Note
AN102 Characterization of a Novel Antibody Drug Conjugate Mimic by Size Exclusion and Hydrophobic Interaction ChromatographyApplication Note
AN109 U/HPLC Workflow Solution for Biosimilar CharacterizationApplication Note
BR09 Solutions for Monoclonal Antibody Analysis and CharacterizationBrochure
DS1038 TSKgel Butyl-NPR ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT03 TSKgel U/HPLC Columns 2019 Product Guide -- HIC ColumnsProduct Catalog
SR074 Separation of Monoclonal Antibodies Using TSKgel HPLC ColumnsSeparation Report
TP164 Detection of protein heterogeneity by HPLCTechnical Presentation
TP181 Detection of protein heterogeneity by HPLC (additional data included)Technical Presentation
TP224 Workflow Solution for the Characterization of Biosimilar using Different Modes of Analytical Chromatography TechniquesTechnical Presentation
TP225 Analysis of Monoclonal Antibody Oxidation Using Hydrophobic Interaction ChromatographyTechnical Presentation
TP228 Characterization of Monoclonal Antibody Heterogeneity from Oxidation and Proteolysis by Analytical Hydrophobic Interaction ChromatographyTechnical Presentation