HPLC Columns

TSKgel Alpha-2500

The TSKgel Alpha-2500 column is packed with rigid, porous, polymer beads which show minimal shrinkage or swelling in polar organic solvents (methanol, acetonitrile, DMSO, isopropanol, THF, and HFIP).  This column is stable in a wide variety of organic solvents at concentrations up to 100%.

The main application area for the TSKgel Alpha-2500 column is the analyses of polymers that are soluble in polar organic solvents up to 10,000 Da. Examples include cellulose derivatives, polyimide, and sodium dodecylsulfate (all in 10 mmol/L LiBr in DMF), cleansing gel in methanol, and degree of saponification of polyvinylalcohol in hexafluoroisopropanol (HFIP).


P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018339TSKgel Alpha-25007Stainless Steel7.8302,074

Product Attributes

Matrix: hydrophilic methacrylate
Particle size (mean): 7 µm
Pore size (mean): 2.5 nm
Exclusion limits
    PEO in H2O: 5,000
    PS in DMF with 
    10 mmol/L LiBr:
1 × 104
    PEG in MeOH with
    10 mmol/L LiBr:
1 × 104




Polyethylene Oxide, Polyethylene Glycol, and Polystyrene Calibration Curves


Columns: TSKgel Alpha columns,
7.8 mm ID × 30 cm
Mobile phase: A. H2O
B. MeOH with 10 mmol/L LiBr
C. DMF with 10 mmol/L LiBr
Flow rate: 1.0 mL/min
Detection: RI
Temperature: A. 25 °C  B. 25 °C  C. 40 °C
Samples: A, B. polyethylene glycol (PEG) and
polyethylene oxide (PEO) standards
C. polystyrene (PS) standards


Analysis of Glyceryl tri(2-ethylhexanoate)




Column: TSKgel Alpha-2500, 7 μm,
7.8 mm ID × 30 cm

Mobile phase: MeOH
Flow rate: 0.5 mL/min
Detection: RI
Temperature: 40 °C
Injection vol.: 50 μL
Samples: glyceryl tri-2-ethylhexanoate
Sample load: 0.10%

Separation of Cleansing Gel



Column: TSKgel Alpha-2500, 7 μm,
7.8 mm ID × 30 cm
Mobile phase: methanol
Flow rate: 0.5 mL/min
Detection: RI
Temperature: 40 °C
Sample: cleansing gel:
1. glyceryl monstearate POE (20)
2. glyceryl tri-2-ethylhexanoate
3. glycerin
4. sorbitol

For additional applications, please see our applications database.


Code #DescriptionLiterature Type
AO01 Gel Filtration Chromatography with TSKgel columns Application Overview
DS1164 TSKgel Alpha-2500 ProductsOperating Conditions and Specifications
IM01 TSKgel Column Instruction ManualInstruction Manual
LCAT01C TSKgel U/HPLC Columns 2019 Product Guide -- SEC, Alpha and SuperAW ColumnsProduct Catalog
PO11 TSKgel Alpha Series Columns for Size Exclusion ChromatographyProduct Overview
SR094 Analyzing Polymers that are Soluble in Polar-Organic Solvents with TSKgel Alpha Series ColumnsSeparation Report
P/NDescriptionParticle Size Housing MaterialID (mm)Length (cm)Price ($) 
0018345TSKgel Alpha-2500-Alpha-M Guard Column for 7.8 mm ID columns13Stainless Steel64789